Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human IL-33 protein
See all IL-33 proteins and peptides
Biological activityActivates Human ST2-dependent NF kappa B pathway.
Purity> 90 % SDS-PAGE.
ab109065 is 0.2 µm filtered.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight18 kDa
Amino acids112 to 270
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab109065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.869% Tris HCl, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CHROMOSOME 9 OPEN READING FRAME 26
FunctionCytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
modificationsThe full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
- Information by UniProt
Pull down assay:
a) pull down of ab109065 by hST2-Fc
b) pull down of hST2-Fc by ab109065.
5 μg of hST2-Fc (or control Fc protein), 2 μg of ab109065 (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5 ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of ab109065 or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab109065 has not yet been referenced specifically in any publications.