Recombinant human IL-4 protein (ab155733)
- Datasheet
- References
- Protocols
Description
-
Product name
Recombinant human IL-4 protein
See all IL-4 proteins and peptides -
Biological activity
Immobilized Human IL-4, Tag Free at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL
-
Purity
> 95 % SDS-PAGE.
ab155733 was lyophilised from 0.22 µm filtered solution. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAAT VLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIMREKYSKCSS -
Predicted molecular weight
15 kDa -
Amino acids
25 to 153
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab155733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Lyophilised -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 6.00
Constituents: 0.39% MES, 5% Trehalose, 1.17% Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
General Info
-
Alternative names
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
see all -
Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
Involvement in disease
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
Images
-
Functional Studies - Recombinant human IL-4 protein (ab155733)ab155733 stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.27-0.49 ng/mL
-
ELISA - Recombinant human IL-4 protein (ab155733)Immobilized ActiveMax® Human IL-4, ab155733, at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL
-
ELISA - Recombinant human IL-4 protein (ab155733)Ab155733 at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha (ab246188) with a linear range of 2-78 ng/mL
-
SDS-PAGE - Recombinant human IL-4 protein (ab155733)Human IL-4 (Tag Free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. -
SDS-PAGE - Recombinant human IL-4 protein (ab155733)SDS-PAGE analysis of reduced ab155733 stained overnight with Coomassie Blue.
Datasheets and documents
References
ab155733 has not yet been referenced specifically in any publications.
