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Our customer purchased ab119444 for direct ELISA.
This protein indicated that reconstitute with acetic acid but coating buffer is alkaline solution of pH 9.6.
So, the customer concerns pH change and possibility to affect results. Check this please.
Asked on May 24 2012
Thank you for contacting us.
I have heard back from the lab. They've advised me that their coating buffer is standard PBS (rather than alkaline carbonate buffers). Thus, your customer may wish to use PBS for coating instead.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on May 24 2012