Bioactive grade

Recombinant human IL-6R protein (ab167742)

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SDS-PAGE - Recombinant human IL-6R protein (ab167742)
  • Functional Studies - Recombinant human IL-6R protein (ab167742)
  • SDS-PAGE - Recombinant human IL-6R protein (ab167742)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human IL-6R protein
    See all IL-6R proteins and peptides

  • Biological activity

    Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 15-60 ng/ml.

  • Purity

    > 90 % SDS-PAGE.
    ab167742 was lyophilized from 0.22 µm filtered solution.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P08887

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      LAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGS HPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQL SCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQ ESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPP ANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWM VKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESR SPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLP

    • Predicted molecular weight

      40 kDa including tags

    • Amino acids

      20 to 365

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 94% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 250 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD 126
    • CD126
    • CD126 antigen
    • gp80
    • IL 6R
    • IL 6R 1
    • IL 6R alpha
    • IL-6 receptor alpha chain
    • IL-6 receptor subunit alpha
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-alpha
    • IL-6RA
    • IL6Q
    • Il6r
    • IL6RA
    • IL6RA_HUMAN
    • IL6RQ
    • Interleukin 6 receptor
    • interleukin 6 receptor, alpha
    • Interleukin-6 receptor subunit alpha
    • Membrane glycoprotein 80
    • MGC30256
    see all

  • Function

    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.

  • Tissue specificity

    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

  • Domain

    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

  • Post-translational
    modifications

    A short soluble form may also be released from the membrane by proteolysis.

  • Cellular localization

    Secreted and Basolateral cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human IL-6R protein (ab167742)
    SDS-PAGE - Recombinant human IL-6R protein (ab167742)
    Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
  • Functional Studies - Recombinant human IL-6R protein (ab167742)
    Functional Studies - Recombinant human IL-6R protein (ab167742)
    Immobilized ActiveMax® Human IL-6, Tag Free at 5 µg/mL (100 µL/well) can bind Human IL-6 R alpha, His Tag with a linear range of 1-16 ng/mL.
  • SDS-PAGE - Recombinant human IL-6R protein (ab167742)
    SDS-PAGE - Recombinant human IL-6R protein (ab167742)
    SDS-PAGE analysis of reduced ab167742 and staining overnight with Coomassie Blue. Protein migrates as 60-70 kDa in reduced SDS-PAGE resulting from glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab167742? Please let us know so that we can cite the reference in this datasheet.

ab167742 has not yet been referenced specifically in any publications.

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