Recombinant human IL2 Receptor beta/p75 protein (Fc Chimera) (ab84068)

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Description

  • Product name

    Recombinant human IL2 Receptor beta/p75 protein (Fc Chimera)
    See all IL2 Receptor beta/p75 proteins and peptides

  • Biological activity

    ab84068 bound to protein A sepharose beads is able to pull down its ligand, IL2.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    HEK 293 cells

  • Accession

    P14784

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      Theoretical Sequence: AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWP DRRRWNQ TCELLPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREG VRWRVMAI QDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYF ERHLEFEART LSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVR VKPLQGEFTTW SPWSQPLAFRTKPAALGIPKVDKKVEPKSCDKTHTCP PCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKF NWYVDGVEVHNAK TKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKV SNKALPAPIEKTIS KAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKG FYPSDIAVEWESNG QPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQ GNVFSCSVMHEALH NHYTQKSLSLSPGK

    • Amino acids

      27 to 237

    • Additional sequence information

      DNA sequence encoding the signal peptide and extracellular domain of human IL 2R beta (aa 1-237) was fused to the Fc region of human IgG1 (aa93-330). Protein expressed in modified human 293 cells.

Specifications

Our Abpromise guarantee covers the use of ab84068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised

  • Additional notes

    ab84068 bound to protein A sepharose beads is able to pull down its ligand, IL2.

     This product was previously labelled as IL2 Receptor beta

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituents: 1% Human serum albumin, 10% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

General Info

  • Alternative names

    • CD122
    • CD122 antigen
    • High affinity IL 2 receptor beta subunit
    • High affinity IL 2 receptor subunit beta
    • High affinity IL-2 receptor subunit beta
    • IL-2 receptor subunit beta
    • IL-2R subunit beta
    • IL-2RB
    • IL15RB
    • IL2 receptor
    • IL2RB
    • IL2RB_HUMAN
    • Interleukin 2 receptor beta
    • Interleukin-2 receptor subunit beta
    • P70 75
    • P70-75
    • P7075
    • p75
    see all

  • Function

    Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.

  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • Cellular localization

    Membrane.
  • Information by UniProt

References

ab84068 has not yet been referenced specifically in any publications.

Publishing research using ab84068? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Does the antibody bind to the extracellular domain of CD122 and is it
known whether that antibody leave the receptor fully functional (IL-2
binding, internalising) once bound?
All the best

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Answer

The ab25227 antibody specificity on the datasheet shows the products does not block the receptor so it means the receptor is fully functional.The epitope of this antibody has not been determined so I am sorry we are unable to confirm which part of domain this antibody binds.

The antibody ab171228; https://www.abcam.com/il2-receptor-beta-tm-beta1-antibody-low-endotoxin-azide-free-ab171228.html is known to inhibit the iL2 to receptor binding.

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Question

Inquiry: Hi, I'm looking to see if it is at all possible to get this material (ab84068) without added albumin. The carrier interferes with the assay I am trying to do. I don't need much, and I am local, and I am willing to have the protein in whatever format is easiest, as long as there is no added protein. Can you give me an idea if this is at all possible? Thanks, Heather

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Answer

Thank you for contacting us.

We cuurrently do not have this product in an albumin free state. The albumin is added by the originating lab as stabilizer and lyophilised prior to shipping. I am sorry for any inconvenience this may cause.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Does this protein retain function, in particular can the IL2 receptor beta still bind protein?

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Answer

This purifed IL-2beta receptor protein does bind IL-2 as shown by its ability to precipitate it whenab84068 is bound to protein A beads in an immunoprecipitation experiment. However it is not a fully functional receptor as ab84068 only contains the extracellular region of the protein.



I hope this helps. If you have any further questions please feel free to contact me again.

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