Recombinant human IL37 protein (Active) (ab224789)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, HPLC

Description

  • Product name

    Recombinant human IL37 protein (Active)
    See all IL37 proteins and peptides

  • Biological activity

    Measured by its ability to bind Interleukin-18 Binding Protein isoform a (IL-18 BPa) in functional ELISA.

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.

  • Expression system

    Escherichia coli

  • Accession

    Q9NZH6

  • Protein length

    Full length protein

  • Animal free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MVHTSPKVKNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFAL ASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLA AQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFE NRKHIEFSFQ PVCKAEMSPS EVSD

    • Predicted molecular weight

      19 kDa

    • Amino acids

      46 to 218

    • Additional sequence information

      This product is the mature full length protein from aa 46 to 218. The propeptide is not included.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab224789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names

    • FIL1
    • FIL1 zeta
    • FIL1(ZETA)
    • FIL1Z
    • IL 1 zeta
    • IL 1F7
    • IL 1F7b (IL 1H4, IL 1H, IL 1RP1)
    • IL 1H4
    • IL 1RP1
    • IL 1X
    • IL 1X protein
    • IL-1 zeta
    • IL-1F7
    • IL-1H
    • IL-1H4
    • IL-1RP1
    • IL-1X
    • IL-37
    • IL1F7
    • IL1F7 (canonical product IL 1F7b)
    • IL1F7_HUMAN
    • IL1H4
    • IL1RP1
    • Interleukin 1 family member 7
    • interleukin 1 family, member 7 (zeta)
    • Interleukin 1 homolog 4
    • Interleukin 1 related protein
    • Interleukin 1 superfamily z
    • Interleukin 1 zeta
    • Interleukin 37
    • Interleukin-1 family member 7
    • Interleukin-1 homolog 4
    • Interleukin-1 zeta
    • Interleukin-1-related protein
    • Interleukin-23
    see all

  • Function

    Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.

  • Tissue specificity

    In general, low constitutive expression, if any, in healthy tissues; high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart.

  • Sequence similarities

    Belongs to the IL-1 family.

  • Post-translational
    modifications

    Proteolytically converted to the mature form by CASP1.

  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions. Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab224789? Please let us know so that we can cite the reference in this datasheet.

ab224789 has been referenced in 1 publication.

  • Zhou P  et al. Interleukin 37 Suppresses M1 Macrophage Polarization Through Inhibition of the Notch1 and Nuclear Factor Kappa B Pathways. Front Cell Dev Biol 8:56 (2020). PubMed: 32117982

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