The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 2E8 cells.
The ED50 <0.2ng/ml, corresponding to a specific activity of >5X106 Unit/mg
< 1.000 Eu/µg
>95% by SDS-PAGE . ab155728 was lyophilized from 0.22 µm filtered solution.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Constituents: 95% PBS, 5% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 250 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.