Recombinant Human INCENP protein (ab135223)

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SDS-PAGE - Recombinant Human INCENP protein (ab135223)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: Functional Studies, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human INCENP protein

    • Purity

      > 90 % SDS-PAGE.
      Affinity purified.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      Q9NQS7

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        AAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDST DDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFK KSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH

      • Predicted molecular weight

        19 kDa including tags

      • Amino acids

        783 to 918

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab135223 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.00
      Preservative: 1.02% Imidazole
      Constituents: 0.002% PMSF, 0.82% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chloride

    General Info

    • Alternative names

      • binds and activates aurora B and C in vivo and in vitro
      • Chromosomal passenger protein
      • INCE_HUMAN
      • INCENP
      • Inner centromere protein
      • Inner centromere protein antigens 135/155kDa
      • Inner centromere protein antigens 135kD 155kD
      • Inner centromere protein INCENP
      see all

    • Function

      Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.

    • Sequence similarities

      Belongs to the INCENP family.

    • Cellular localization

      Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human INCENP protein (ab135223)
      SDS-PAGE - Recombinant Human INCENP protein (ab135223)
      SDS-PAGE analysis of ab135223.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab135223? Please let us know so that we can cite the reference in this datasheet.

    ab135223 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Inquiry: Hello, I would like to know whether it is possible to use this INCENP in vitro for activation of Aurora B kinase? The applications tested by Abcam include "functional studies", but I am not sure what it means.

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    Answer




    I am happy to confirm that ab135223 can be used for Aurora B and Aurora C activation in vitro.


    Recombinant human INCENP (783-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.


    Maybe these publications will be helpful when establishing the binding/ activation/ kinase assay for INCENP and Aurora B kinase:


    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917241/


    http://www.sciencedirect.com/science/article/pii/S1097276505012244


    http://www.jbc.org/content/277/31/27577.long


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