Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, Functional Studies
Description
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Product name
Recombinant human Indoleamine 2, 3-dioxygenase protein (Active)
See all Indoleamine 2, 3-dioxygenase proteins and peptides -
Biological activity
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25°C.
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Purity
> 90 % SDS-PAGE.
ab219439 was purified by using conventional chromatography techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMAHAMENSWTISKEYHIDEEVGFALPN PQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQR LARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYA DCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASA IKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFF SVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLL GIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAG LREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAK GTGGTDLMNFLKTVRSTTEKSLLKEG -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 403 -
Tags
His tag N-Terminus -
Additional sequence information
(NP_002155).
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Associated products
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Related Products
- Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (ab211017)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305)
- Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab219439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
Functional Studies
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 3-dioxygenase
- I23O1_HUMAN
- IDO
see all -
Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab219439 has not yet been referenced specifically in any publications.