Bioactive grade

Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)

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SDS-PAGE - Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS, Functional Studies

    Description

    • Product name

      Recombinant human Indoleamine 2, 3-dioxygenase protein (Active)
      See all Indoleamine 2, 3-dioxygenase proteins and peptides

    • Biological activity

      Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25°C.

    • Purity

      > 90 % SDS-PAGE.
      ab219439 was purified by using conventional chromatography techniques.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P14902

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMAHAMENSWTISKEYHIDEEVGFALPN PQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQR LARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYA DCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASA IKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFF SVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLL GIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAG LREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAK GTGGTDLMNFLKTVRSTTEKSLLKEG

      • Predicted molecular weight

        48 kDa including tags

      • Amino acids

        1 to 403

      • Tags

        His tag N-Terminus

      • Additional sequence information

        (NP_002155).

    Specifications

    Our Abpromise guarantee covers the use of ab219439 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

      Functional Studies

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.02% DTT

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • 3-dioxygenase
      • I23O1_HUMAN
      • IDO
      • IDO 1
      • IDO-1
      • IDO1
      • INDO
      • indolamine 2,3 dioxygenase
      • Indole 2 3 dioxygenase
      • indoleamine 2 3 dioxygenase
      • indoleamine 2 3 dioxygenase 1
      • Indoleamine 2,3-dioxygenase 1
      • Indoleamine pyrrole 2 3 dioxygenase
      • Indoleamine-pyrrole 2
      see all

    • Function

      Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.

    • Pathway

      Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.

    • Sequence similarities

      Belongs to the indoleamine 2,3-dioxygenase family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)
      SDS-PAGE - Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)

      15% SDS-PAGE analysis of ab219439 (3µg).   

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab219439? Please let us know so that we can cite the reference in this datasheet.

    ab219439 has not yet been referenced specifically in any publications.

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