Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, Functional Studies
Product nameRecombinant human Indoleamine 2, 3-dioxygenase protein (Active)
See all Indoleamine 2, 3-dioxygenase proteins and peptides
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25°C.
Purity> 90 % SDS-PAGE.
ab219439 was purified by using conventional chromatography techniques.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHHSSGLVPRGSHMGSMAHAMENSWTISKEYHIDEEVGFALPN PQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQR LARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYA DCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASA IKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFF SVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLL GIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAG LREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAK GTGGTDLMNFLKTVRSTTEKSLLKEG
Predicted molecular weight48 kDa including tags
Amino acids1 to 403
TagsHis tag N-Terminus
Additional sequence information(NP_002155).
- Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (ab211017)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305)
- Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab219439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.02% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionCatalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
PathwayAmino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the indoleamine 2,3-dioxygenase family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219439 has not yet been referenced specifically in any publications.