Bioactive grade

Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)

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Description

  • Product name

    Recombinant human Indoleamine 2, 3-dioxygenase protein (Active)
    See all Indoleamine 2, 3-dioxygenase proteins and peptides

  • Biological activity

    Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25°C.

  • Purity

    > 90 % SDS-PAGE.
    ab219439 was purified by using conventional chromatography techniques.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P14902

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMAHAMENSWTISKEYHIDEEVGFALPN PQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQR LARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYA DCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASA IKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFF SVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLL GIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAG LREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAK GTGGTDLMNFLKTVRSTTEKSLLKEG

    • Predicted molecular weight

      48 kDa including tags

    • Amino acids

      1 to 403

    • Tags

      His tag N-Terminus

    • Additional sequence information

      (NP_002155).

Specifications

Our Abpromise guarantee covers the use of ab219439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

    Functional Studies

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 20% Glycerol, 0.02% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 3-dioxygenase
    • I23O1_HUMAN
    • IDO
    • IDO 1
    • IDO-1
    • IDO1
    • INDO
    • indolamine 2,3 dioxygenase
    • Indole 2 3 dioxygenase
    • indoleamine 2 3 dioxygenase
    • indoleamine 2 3 dioxygenase 1
    • Indoleamine 2,3-dioxygenase 1
    • Indoleamine pyrrole 2 3 dioxygenase
    • Indoleamine-pyrrole 2
    see all

  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.

  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.

  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)
    SDS-PAGE - Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)

    15% SDS-PAGE analysis of ab219439 (3µg).   

References

ab219439 has not yet been referenced specifically in any publications.

Publishing research using ab219439? Please let us know so that we can cite the reference in this datasheet.

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