Recombinant Human Inhibin beta A protein (ab119449)

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Overview

Description

  • Nature

    Recombinant

  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P08476

    • Species

      Human

    • Molecular weight

      14 kDa including tags

    • Amino acids

      311 to 426

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab119449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    HPLC

  • Purity

    > 95 % SDS-PAGE.
    Purity is >95% by SDS PAGE and HPLC analysis.

  • Form

    Liquid

  • Additional notes

    ab119449 should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 49% Tris buffered saline, 50% Glycerol

General Info

  • Alternative names

    • Activin beta-A chain
    • EDF
    • Erythroid differentiation factor
    • Erythroid differentiation protein
    • Follicle stimulating hormone releasing protein
    • FRP
    • FSH releasing protein
    • INHBA
    • INHBA_HUMAN
    • Inhibin beta A chain
    • Inhibin beta A subunit
    • Inhibin, beta 1
    • Inhibin, beta A (activin A, activin AB alpha polypeptide)
    see all

  • Function

    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab119449 has not yet been referenced specifically in any publications.

Publishing research using ab119449? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I will be using recombinant human inhibin A (dimer, bto alpha and beta A chains with his tag) as calibrator. Just to confirm suitable antibodies Anti inhibin beta A (ab4241) for capture and anti inhibin alpha (ab47720) for reporter. Ok? Best regards,

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Answer

Thank you for your email. Ab4241 is raised against Inhibin beta A chain and ab47720 is raised against Inhibin alpha cahin. Theoretically these antibodies can be used in sELISA experiment. I am however unable to confirm because we haven't tested these antibodies together. We do not have data to prove the assumption of using this abs together. You may need to optimize the protocol yourselves. TheInhibin beta A chain and Inhibin alpha protein sequence does not match so the antibodies against these proteins can be used in sELISA. Please also be advised that ab4241 and ab47720 are not conjugated antibodies; any of these can be used as detection or capture ab however you will need a tertiary conjugated antibody for detection. Now if you are planning to use ab4241 and ab47720 please be aware that both of these are mouse monoclonal; the tertiary anti mouse antibody will bind to both capture and detections ab thereforewill givehigh background intensity. This situation can be overcome by using one rabbit and other mouse antibody. I suggest using ab93847, ab97705 or ab113489 as substitution for ab4241 or ab35106, ab81234 as subs for ab47720. Finally, if you choose 2 antibodies taking in consideration of the above information I sent; I can then provide you2 Abreview discount codes for using these antibodies together in sELISA. You can get 2 antibodies freeof charge in your next purchase with these discount codes. To activate the discount codes you have to use the products together thereby submitting an Abreview. Please click the link below for further update; https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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Question

I will be using recombinant human inhibin A (dimer, bto alpha and beta A chains with his tag) as calibrator. Just to confirm suitable antibodies Anti inhibin beta A (ab4241) for capture and anti inhibin alpha (ab47720) for reporter. Ok? Best regards,

Read More
Answer

I have received your inquiry and have started working on it. I am seeking more information from lab regarding your inquiry. I will get back to you with the information soon. Many thanks for having patience!

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Question

Can Inhibin beta A protein (His tag) (ab119449) Bu used as a calibrator in an Elisa Assay using your 4A2F2 and 4E2 antibodies Thankyou Best regards,

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Answer

Thank you for contacting us. The product ab119449 is an Inhibin beta A protein however the antibodies are ab105927 (4A2F2)and ab47720 (4E2) are anti Inihibin alpha so these antibodies cannot used in conjunction with ab119449. You may however consider using ab4241 or ab93847 with ab119449. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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