Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Product nameRecombinant human Insulin protein (Active)
See all Insulin proteins and peptides
ab123768 is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.
Purity> 98 % SDS-PAGE.
> 98% SDS-PAGE. > 98% HPLC. Recombinant Insulin is purified by proprietary chromatographic techniques.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceGIVEQCCTSIC SLYQLENYCN FVNQHL CGSHLVEALY LVCGERGFFY TPKT
Predicted molecular weight6 kDa
Additional sequence informationTwo chain, non-glycosylated polypeptide chain. (aa 25-54 and 90-110)
Our Abpromise guarantee covers the use of ab123768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 4772 Insulin, human recombinant (E. coli). 4772-5 is the same size as the 5 mg size of ab123768.
Endotoxin Level: <0.1 ng/µg of Insulin.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C. Store under desiccating conditions.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 0.01 N HCl. The solution can then be diluted to other aqueous buffers. Upon reconstitution, ab123768 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store aliquots at –20°C or –70°C. Avoid freeze-thaw cycles.
FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesBelongs to the insulin family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123768 has been referenced in 1 publication.
- Cao L et al. Design of protein-binding proteins from the target structure alone. Nature 605:551-560 (2022). PubMed: 35332283