Description

  • Product name

    Recombinant human Insulin protein (Active)
    See all Insulin proteins and peptides
  • Biological activity

    ab123768 is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.
  • Purity

    > 98 % SDS-PAGE.
    > 98% HPLC Endotoxin Level: <0.1 ng/µg of Insulin. Recombinant Insulin is purified by proprietary chromatographic techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      GIVEQCCTSIC SLYQLENYCN FVNQHL CGSHLVEALY LVCGERGFFY TPKT
    • Predicted molecular weight

      6 kDa
    • Additional sequence information

      Two chain, non-glycosylated polypeptide chain. (aa 25-54 and 90-110)

Specifications

Our Abpromise guarantee covers the use of ab123768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C. Store under desiccating conditions.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in 0.01 N HCl. The solution can then be diluted to other aqueous buffers. Upon reconstitution, ab123768 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store aliquots at –20°C or –70°C. Avoid freeze-thaw cycles.

General Info

  • Alternative names

    • IDDM
    • IDDM1
    • IDDM2
    • ILPR
    • ins
    • INS_HUMAN
    • Insulin A chain
    • Insulin B chain
    • IRDN
    • MODY10
    • Preproinsulin
    • Proinsulin
    • Proinsulin precursor
    see all
  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Under reducing conditions and stained with Coomassie Blue.

    Lane 1: 2 µg Human Insulin.

    Lane 2: 5 µg Human Insulin.

    Lane 3: 10 µg Human Insulin.

    Human recombinant insulin has a predicted MW of 5.81 kDa

References

ab123768 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
ELISA
The human insulin protein (ab123768) was used in a sandwich ELISA from 250 ng/ml down to 3.9 ng/ml using the D6C4 anti insulin+proinsulin mouse capture antibody (ab8304) and the D3E7 -biotinylated anti Insulin+pro insulin mouse detection antibody (ab20756).
The human insulin standard was detected by the mouse antibody set described above.

Abcam user community

Verified customer

Submitted May 01 2017

Abreviews
Application
ELISA
Sandwich ELISA was set up using human purified insulin for standard curve, set up from 1.5μg/mL protein in a 1:2 serial dilution. Ab14042 used at 2μg/mL as capture antibody and Ab6995 used at 1:50 in a 1:4 serial dilution used for the detection antibody. Anti-mouse HRP conjugated secondary antibody used at 1:20,000.

Ms. Vruti Patel

Verified customer

Submitted Dec 17 2014

Answer

We do offer a number of active insulin proteins for use as standard including https://www.abcam.com/insulin-protein-active-ab73797.html (25mg size) or https://www.abcam.com/insulin-protein-active-ab123768.html(5mg size). Abcam also carries a number of products which can effect insulin and related processes such as Mitiglinide hemicalcium salt (https://www.abcam.com/mitiglinide-hemicalcium-salt-ab143666.html) which can stimulate insulin secretion, HNMPA-(AM)3 (https://www.abcam.com/hnmpa-am3-ab141567.html) a Insulin receptor tyrosine kinase inhibitor and Chromium picolinate (https://www.abcam.com/chromium-picolinate-ab142703.html) which aids in enhancing the function of insulin.

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