Description

  • Product name

    Recombinant human Insulin protein (Active)
    See all Insulin proteins and peptides
  • Biological activity

    28 units/mg

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      GIVEQCCTSI CSLYQLENYC NFVNQHLCGS HLVEALYLVC GERGFFYTPK T
    • Predicted molecular weight

      6 kDa
    • Additional sequence information

      ab73797 is a two chain (aa 90-110 insulin A, aa 25-54 insulin B), non-glycosylated polypeptide chain.

Specifications

Our Abpromise guarantee covers the use of ab73797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute the lyophilized Insulin in sterile 0.005N HCl to not more than 1 mg/ml.

General Info

  • Alternative names

    • IDDM
    • IDDM1
    • IDDM2
    • ILPR
    • ins
    • INS_HUMAN
    • Insulin A chain
    • Insulin B chain
    • IRDN
    • MODY10
    • Preproinsulin
    • Proinsulin
    • Proinsulin precursor
    see all
  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab73797 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

We do offer a number of active insulin proteins for use as standard including https://www.abcam.com/insulin-protein-active-ab73797.html (25mg size) or https://www.abcam.com/insulin-protein-active-ab123768.html(5mg size). Abcam also carries a number of products which can effect insulin and related processes such as Mitiglinide hemicalcium salt (https://www.abcam.com/mitiglinide-hemicalcium-salt-ab143666.html) which can stimulate insulin secretion, HNMPA-(AM)3 (https://www.abcam.com/hnmpa-am3-ab141567.html) a Insulin receptor tyrosine kinase inhibitor and Chromium picolinate (https://www.abcam.com/chromium-picolinate-ab142703.html) which aids in enhancing the function of insulin.

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Answer

Thank you for your phone call.

DISCOUNT CODE: xxx for testing ab79477 in ELISA and sELISA
DISCOUNT CODE: xxx for testing ab65454 in sELISA
DISCOUNT CODE: xxx for testing ab73797 in ELISA and sELISA

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab79477, ab65454 and ab73797 in ELISA and/or sELISA. Each code will give you either 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit an Abreview for ELISA and sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. Each code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. You can ask any questions to us we are happy to help. We can only guarantee these proteins acting as inducers in functional assays in human cells. These proteins are tested only with human cells. The RAT insulin 1, 2 and human insulin sequence is 83% similar so theoretically these proteins may induce cellular activities in Rat cells also. However we are unable to confirm it because we don’t have tested data of rat cells. If you would like to use these proteins with rat samples then let me know I will be happy to create a discount code for you. The discount code will give you, next purchase of 1 protein product free of charge. I would however also need the information of assay you would like to perform.  I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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