Bioactive grade

Recombinant human Integrin alpha 4/CD49D + Integrin beta 7 protein (Tagged) (Biotin) (ab246163)

Description

  • Product name

    Recombinant human Integrin alpha 4/CD49D + Integrin beta 7 protein (Tagged) (Biotin)
  • Biological activity

    Immobilized Anti-alpha4 unit at 1 μg/mL (100 μL/well) can bind ab246163 with a linear range of 4-63 ng/mL (QC tested).

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Predicted molecular weight

      113 kDa including tags
    • Amino acids

      34 to 977
    • Tags

      His tag C-Terminus , Avi tag C-Terminus
    • Additional sequence information

      Extracellular domain. Acidic tail.
    • Amino Acid Sequence 2

    • Species

      Human
    • Predicted molecular weight

      82 kDa
    • Amino acids

      20 to 723
    • Additional sequence information

      Extracellular domain. Basic tail.
  • Conjugation

    Biotin

Specifications

Our Abpromise guarantee covers the use of ab246163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Lyophilised
  • Additional notes

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.

    pH: 7.50
    Constituents: 5% Trehalose, 0.61% Tris, 0.87% Sodium chloride

    0.22 µm filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long-term storage.

General Info

  • Cellular localization

    Integrin alpha 4/CD49D: Membrane. Integrin beta 7: Membrane.

Images

  • SDS page analysis of ab246163 under reducing conditions. The reducing (R) protein migrates as 110 kDa and 130 kDa (ITGA4) and 60-70 kDa (ITGB7) respectively due to glycosylation.

  • Immobilized Anti-alpha4 unit at 1 μg/mL (100 μL/well) can bind ab246163 with a linear range of 4-63 ng/mL (QC tested).

References

ab246163 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab246163.
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