Bioactive grade

Recombinant human Interferon alpha 1 protein (Active) (ab48750)

Description

  • Product name

    Recombinant human Interferon alpha 1 protein (Active)
    See all Interferon alpha 1 proteins and peptides
  • Biological activity

    Interferon alpha 1 (ab48750) has the activity of 4 X 105 unit/mg (or 4 X 105 unit/ml).

    This activity has been tested by evaluating anti-proliferation with Daudi cells. For Further information see Kim et al., 2009.

  • Purity

    > 95 % SDS-PAGE.
    This protein was purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      CDLPETHSLD NRRTLMLLAQ MSRISPSSCL MDRHDFGFPQ EEFDGNQFQK APAISVLHEL IQQIFNLFTT KDSSAAWDED LLDKFCTELY QQLNDLEACV MQEERVGETP LMNADSILAV KKYFRRITLY LTEKKYSPCA WEVVRAEIMR SLSLSTNLQE RLRRKE
    • Predicted molecular weight

      20 kDa
    • Amino acids

      24 to 189
    • Additional sequence information

      Full length mature protein, without the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab48750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Sandwich ELISA

  • Form

    Liquid
  • Additional notes

    Concentration determined by Bradford assay.

    Endotoxin levels determined by LAL method.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • IFL
    • IFN
    • IFN alpha
    • IFN alpha 1/13
    • IFN alpha 1b
    • IFN alphaD
    • IFN-alpha-1/13
    • IFNA@
    • IFNA1
    • IFNA1_HUMAN
    • IFNA13
    • Interferon alpha 1
    • Interferon alpha-1/13
    • Interferon alpha-D
    • Interferon alpha1
    • Interferon, alpha 13
    • LeIF D
    see all
  • Function

    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similarities

    Belongs to the alpha/beta interferon family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Standard curve for Interferon alpha 1 (Analyte: ab48750); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab20200 at 5µg/ml and Detector Antibody ab84257 at 0.5µg/ml.

  • 3ug of reduced ab48750 on SDS-PAGE, stained with Coomassie Blue. 

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. INF is a cell factor, interferon alpha. We have following products in catalogue related to INF alpha protein ab9661 ab48750 ab73124 ab84997 ab95135 ab87926 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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