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Immunology Innate Immunity Cytokines Interferons
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Recombinant human Interferon alpha 2 protein (ab151900)

  • Datasheet
  • SDS
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Key features and details

  • Expression system: Pichia pastoris
  • Purity: > 98% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

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Description

  • Product name

    Recombinant human Interferon alpha 2 protein
    See all Interferon alpha 2 proteins and peptides
  • Biological activity

    Specific Activity is 5.0 x 106 IU/mg as determined by a viral resistance assay using VSV-WISH cells.
  • Purity

    > 98 % SDS-PAGE.
    Purity is greater than 98% as determined by SEC-HPLC and reducing SDS-PAGE.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Pichia pastoris
  • Accession

    P01563
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFA KTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNE CFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFY APELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDL LECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLA KTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGE YKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAE DYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPK EFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDD FAAFVEKCCKADDKETCFAEEGKKLVAASQAALGLCDLPQTHSLGSRRTL MLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFN LFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDS ILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
    • Predicted molecular weight

      86 kDa
    • Amino acids

      24 to 188
    • Additional sequence information

      Recombinant Interferon alpha 2 linked to human albumin.

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab151900 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      HPLC

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      pH: 7.20
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilized protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml.
      Reconstituted protein solution can be stored at 4-7°C for 2-7 days. For long term storage aliquot and store at < -20°C.

    General Info

    • Alternative names

      • Alpha 2a interferon
      • IFN
      • IFN alpha 2
      • IFN alphaA
      • IFN-alpha-2
      • IFNA
      • Ifna2
      • IFNA2_HUMAN
      • IFNA2B
      • Interferon
      • Interferon alpha 2a
      • Interferon alpha 2b
      • Interferon alpha A
      • Interferon alpha-2
      • Interferon alpha-A
      • LeIF A
      • LeIFA
      • MGC125764
      • MGC125765
      see all
    • Function

      Produced by macrophages, IFN-alpha have antiviral activities.
    • Sequence similarities

      Belongs to the alpha/beta interferon family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01563 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab151900? Please let us know so that we can cite the reference in this datasheet.

    ab151900 has not yet been referenced specifically in any publications.

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