Recombinant Human IRAK-1 protein (ab125533)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      P51617
    • Species
      Human
    • Molecular weight
      74 kDa including tags
    • Amino acids
      194 to 712

Specifications

Our Abpromise guarantee covers the use of ab125533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 70 % Densitometry.
    Purity determined to be >70% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names
    • AA48924
    • Il1rak
    • Interleukin 1 receptor associated kinase 1
    • Interleukin-1 receptor-associated kinase 1
    • IRAK
    • IRAK-1
    • Irak1
    • IRAK1-S
    • IRAK1_HUMAN
    • mPLK
    • OTTHUMP00000026014
    • OTTHUMP00000026015
    • OTTHUMP00000026020
    • OTTHUMP00000180621
    • Pelle
    • Pelle homolog
    • Pelle-like protein kinase
    • Plpk
    see all
  • Function
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • Tissue specificity
    Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human IRAK-1 protein (ab125533)
    SDS-PAGE - Recombinant Human IRAK-1 protein (ab125533)
    SDS-PAGE analysis of ab125533.

References

ab125533 has not yet been referenced specifically in any publications.

Publishing research using ab125533? Please let us know so that we can cite the reference in this datasheet.

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