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  1. Link

    recombinant-human-irak-1-protein-ab125533.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Recombinant Human IRAK-1 protein (ab125533)

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  • SDS-PAGE - Recombinant Human IRAK-1 protein (ab125533)
  • Datasheet
  • References
  • Protocols

Description

  • Product name

    Recombinant Human IRAK-1 protein
  • Purity

    > 70 % Densitometry.
    Purity determined to be >70% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P51617
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      74 kDa including tags
    • Amino acids

      194 to 712

Associated products

  • Related Products

    • Anti-IRAK-1 antibody (ab238)

Specifications

Our Abpromise guarantee covers the use of ab125533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • AA48924
    • Il1rak
    • Interleukin 1 receptor associated kinase 1
    • Interleukin-1 receptor-associated kinase 1
    • IRAK
    • IRAK-1
    • Irak1
    • IRAK1-S
    • IRAK1_HUMAN
    • mPLK
    • OTTHUMP00000026014
    • OTTHUMP00000026015
    • OTTHUMP00000026020
    • OTTHUMP00000180621
    • Pelle
    • Pelle homolog
    • Pelle-like protein kinase
    • Plpk
    see all
  • Function

    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • Tissue specificity

    Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Target information above from: UniProt accession P51617 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human IRAK-1 protein (ab125533)
    SDS-PAGE - Recombinant Human IRAK-1 protein (ab125533)
    SDS-PAGE analysis of ab125533.

Datasheets and documents

    • Datasheet
  • References

    ab125533 has not yet been referenced specifically in any publications.

    Publishing research using ab125533? Please let us know so that we can cite the reference in this datasheet.

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