Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human IRAK-1 protein (Active)
See all IRAK-1 proteins and peptides
Specific activity ≥40 pmol/min/µg.
Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceSVSLLQGARP FPFCWPLCEI SRGTHNFSEE LKIGEGGFGC VYRAVMRNTV YAVKRLKENA DLEWTAVKQS FLTEVEQLSR FRHPNIVDFA GYCAQNGFYC LVYGFLPNGS LEDRLHCQTQ ACPPLSWPQR LDILLGTARA IQFLHQDSPS LIHGDIKSSN VLLDERLTPK LGDFGLARFS RFAGSSPSQS SMVARTQTVR GTLAYLPEEY IKTGRLAVDT DTFSFGVVVL ETLAGQRAVK THGARTKYLK DLVEEEAEEA GVALRSTQST LQAGLAADAW AAPIAMQIYK KHLDPRPGPC PPELGLGLGQ LACCCLHRRA KRRPPMTQVY ERLEKLQAVV AGVPG
Predicted molecular weight40 kDa including tags
Amino acids186 to 530
TagsHis tag N-Terminus
Additional sequence informationGenbank: NM_001569
Our Abpromise guarantee covers the use of ab271553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 0.34% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Interleukin 1 receptor associated kinase 1
FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Tissue specificityIsoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271553 has not yet been referenced specifically in any publications.