Key features and details
- Expression system: Insect cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameRecombinant human IRAK4 protein (Active)
See all IRAK4 proteins and peptides
The specific activity of IRAK4 was determined to be 41.6 nmol/min/mg.
Purity> 80 % SDS-PAGE.
Expression systemInsect cells
Protein lengthProtein fragment
SequenceAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSP ENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKG YVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSS DGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLH ENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTA YMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEI EDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQL LQEMTAS
Predicted molecular weight52 kDa including tags
Actual molecular weight68 kDa including tags
Amino acids104 to 460
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab268681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- IL-1 receptor-associated kinase 4
- Interleukin 1 receptor associated kinase 4 mutant form 1
- Interleukin-1 receptor-associated kinase 4
FunctionRequired for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
Involvement in diseaseDefects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268681 has not yet been referenced specifically in any publications.