Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, Avi tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human IRE1 protein (Active)
See all IRE1 proteins and peptides
Assay was done in a kinase buffer using MBP (NEB, 0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 mins. The amount of ATP transferred was calculated using Kinase-Glo reagent.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceHHHHHHHHHHGGGENLYFQGGLNDIFEAQKIEWHEHQQQQLQHQQFQKEL EKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRAS NHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEG TIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEK DRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLN IVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPG TEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQA NILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFW SLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQ TDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYF TSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL
Predicted molecular weight62 kDa
Amino acids469 to 977
TagsHis tag N-Terminus , Avi tag N-Terminus
Additional sequence informationN-terminal Tev protease tag.
Our Abpromise guarantee covers the use of ab271544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 2.04% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Endoplasmic reticulum (ER) to nucleus signalling 1
- Endoplasmic reticulum to nucleus signaling 1
- Endoplasmic reticulum-to-nucleus signaling 1
FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271544 has not yet been referenced specifically in any publications.