Recombinant human IRE1 protein (Active) (ab271544)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, Avi tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IRE1 protein (Active)
See all IRE1 proteins and peptides -
Biological activity
Assay was done in a kinase buffer using MBP (NEB, 0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 mins. The amount of ATP transferred was calculated using Kinase-Glo reagent.
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Purity
>= 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
HHHHHHHHHHGGGENLYFQGGLNDIFEAQKIEWHEHQQQQLQHQQFQKEL EKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRAS NHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEG TIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEK DRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLN IVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPG TEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQA NILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFW SLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQ TDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYF TSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL -
Predicted molecular weight
62 kDa -
Amino acids
469 to 977 -
Tags
His tag N-Terminus , Avi tag N-Terminus -
Additional sequence information
N-terminal Tev protease tag.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab271544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 2.04% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Endoplasmic reticulum (ER) to nucleus signalling 1
- Endoplasmic reticulum to nucleus signaling 1
- Endoplasmic reticulum-to-nucleus signaling 1
see all -
Function
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
Tissue specificity
Ubiquitously expressed. High levels observed in pancreatic tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab271544 has not yet been referenced specifically in any publications.