Recombinant human IRE1b protein (ab190994)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IRE1b protein -
Biological activity
The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.
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Purity
> 95 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
KQ AQPLDDPEAE QLTVVGKISF NPKDVLGRGA GGTFVFRGQF EGRAVAVKRL LRECFGLVRR EVQLLQESDR HPNVLRYFCT ERGPQFHYIA LELCRASLQE YVENPDLDRG GLEPEVVLQQ LMSGLAHLHS LHIVHRDLKP GNILITGPDS QGLGRVVLSD FGLCKKLPAG RCSFSLHSGI PGTEGWMAPE LLQLLPPDSP TSAVDIFSAG CVFYYVLSGG SHPFGDSLYR QANILTGAPC LAHLEEEVHD KVVARDLVGA MLSPLPQPRP SAPQVLAHPF FWSRAKQLQF FQDVSDWLEK ESEQEPLVRA LEAGGCAVVR DNWHEHISMP LQTDLRKFRS YKGTSVRDLL RAVRNKKHHY RELPVEVRQA LGQVPDGFVQ YFTNRFPRLL LHTHRAMRSC ASESLFLPYY PPDSEARRPC PGATGR -
Predicted molecular weight
80 kDa including tags -
Amino acids
499 to 926 -
Tags
proprietary tag N-Terminus -
Additional sequence information
NM_033266.
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab190994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Endoplasmic reticulum to nucleus signaling 2
- Endoplasmic reticulum-to-nucleus signaling 2
- Endoribonuclease
see all -
Function
Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
Images
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The specific activity of IRE1b (ab190994) was determined to be 2.4 nmol/min/mg as per activity assay protocol and was equivalent to 2.3 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab190994
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SDS PAGE analysis of ab190994
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The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.
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The purity of ab190994 was determined to be >95% by densitometry, approx MW ~80 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190994 has not yet been referenced specifically in any publications.