Recombinant Human IRS1 protein (ab70538)

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Description

  • Product name

    Recombinant Human IRS1 protein
    See all IRS1 proteins and peptides

  • Expression system

    Insect cells

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Amino acids

      600 to 1245

Specifications

Our Abpromise guarantee covers the use of ab70538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride

General Info

  • Alternative names

    • HIRS 1
    • HIRS1
    • Insulin receptor substrate 1
    • IRS 1
    • IRS-1
    • IRS1
    • IRS1_HUMAN
    • OTTHUMP00000164234
    see all

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.

  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human IRS1 protein (ab70538)
    SDS-PAGE - Recombinant Human IRS1 protein (ab70538)
    SDS-PAGE showing IRS1 at approximately 118kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab70538? Please let us know so that we can cite the reference in this datasheet.

ab70538 has not yet been referenced specifically in any publications.

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