Recombinant human ITK/EMT protein (ab60300)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human ITK/EMT protein
See all ITK/EMT proteins and peptides -
Biological activity
Specific activity: 58 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
53 kDa -
Amino acids
352 to 620 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant fragment, corresponding to amino acids 352-end of Human ITK.
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as ITK.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EMT
- Homolog of mouse T cell itk/tsk
- IL 2 inducible T cell kinase
see all -
Function
Plays a role in T-cell proliferation and differentiation. -
Tissue specificity
T-cell lines and natural killer cell lines. -
Involvement in disease
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localization
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
Images
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Functional Studies - Recombinant human ITK/EMT protein (ab60300)The specific activity of ITK/EMT (ab60300) was determined to be 35 nmol/min/mg as per activity assay protocol and was equivalent to 50 nmol/min/mg as per radiometric assay -
SDS-PAGE - Recombinant human ITK/EMT protein (ab60300)SDS PAGE analysis of ab60300 -
Functional Studies - Recombinant human ITK/EMT protein (ab60300)Sample Kinase assay plot. -
SDS-PAGE - Recombinant human ITK/EMT protein (ab60300)ab60300 on SDS-PAGE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab60300 has not yet been referenced specifically in any publications.
