Recombinant human ITK/EMT protein (ab60300)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human ITK/EMT protein
See all ITK/EMT proteins and peptides -
Biological activity
Specific activity: 58 nmol/min/mg.
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Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
53 kDa -
Amino acids
352 to 620 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant fragment, corresponding to amino acids 352-end of Human ITK.
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as ITK.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EMT
- Homolog of mouse T cell itk/tsk
- IL 2 inducible T cell kinase
see all -
Function
Plays a role in T-cell proliferation and differentiation. -
Tissue specificity
T-cell lines and natural killer cell lines. -
Involvement in disease
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localization
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
Images
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Functional Studies - Recombinant human ITK/EMT protein (ab60300)The specific activity of ITK/EMT (ab60300) was determined to be 35 nmol/min/mg as per activity assay protocol and was equivalent to 50 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human ITK/EMT protein (ab60300)SDS PAGE analysis of ab60300 -
Functional Studies - Recombinant human ITK/EMT protein (ab60300)Sample Kinase assay plot. -
SDS-PAGE - Recombinant human ITK/EMT protein (ab60300)ab60300 on SDS-PAGE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab60300 has not yet been referenced specifically in any publications.
