• Product name

    Recombinant human ITK/EMT protein
    See all ITK/EMT proteins and peptides
  • Biological activity

    Specific activity: 58 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

  • Nature

    • Species

    • Predicted molecular weight

      53 kDa
    • Amino acids

      352 to 620
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      Recombinant fragment, corresponding to amino acids 352-end of Human ITK.

Associated products


Our Abpromise guarantee covers the use of ab60300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies


  • Form

  • Additional notes

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

    Previously labelled as ITK. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • EMT
    • Homolog of mouse T cell itk/tsk
    • IL 2 inducible T cell kinase
    • IL2 inducible T cell kinase
    • Interleukin 2 inducible T cell kinase
    • Itk
    • Kinase EMT
    • LPFS1
    • LYK
    • MGC126257
    • MGC126258
    • PSCTK 2
    • PSCTK2
    • T cell specific kinase
    • T-cell-specific kinase
    • TSK
    • Tyrosine protein kinase
    • Tyrosine protein kinase ITK/TSK
    • Tyrosine protein kinase Lyk
    • Tyrosine-protein kinase ITK/TSK
    • Tyrosine-protein kinase Lyk
    see all
  • Function

    Plays a role in T-cell proliferation and differentiation.
  • Tissue specificity

    T-cell lines and natural killer cell lines.
  • Involvement in disease

    Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
  • Information by UniProt


  • The specific activity of ITK/EMT (ab60300) was determined to be 35 nmol/min/mg as per activity assay protocol and was equivalent to 50 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab60300
  • Sample Kinase assay plot.
  • ab60300 on SDS-PAGE.


ab60300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab60300.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact

Sign up