Description

  • Product name

    Recombinant human ITK/EMT protein
    See all ITK/EMT proteins and peptides
  • Biological activity

    Specific activity: 58 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      53 kDa
    • Amino acids

      352 to 620
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      Recombinant fragment, corresponding to amino acids 352-end of Human ITK.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

    Previously labelled as ITK. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • EMT
    • Homolog of mouse T cell itk/tsk
    • IL 2 inducible T cell kinase
    • IL2 inducible T cell kinase
    • Interleukin 2 inducible T cell kinase
    • Itk
    • ITK_HUMAN
    • Kinase EMT
    • LPFS1
    • LYK
    • MGC126257
    • MGC126258
    • PSCTK 2
    • PSCTK2
    • T cell specific kinase
    • T-cell-specific kinase
    • TSK
    • Tyrosine protein kinase
    • Tyrosine protein kinase ITK/TSK
    • Tyrosine protein kinase Lyk
    • Tyrosine-protein kinase ITK/TSK
    • Tyrosine-protein kinase Lyk
    see all
  • Function

    Plays a role in T-cell proliferation and differentiation.
  • Tissue specificity

    T-cell lines and natural killer cell lines.
  • Involvement in disease

    Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
  • Information by UniProt

Images

  • The specific activity of ITK/EMT (ab60300) was determined to be 35 nmol/min/mg as per activity assay protocol and was equivalent to 50 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab60300
  • Sample Kinase assay plot.
  • ab60300 on SDS-PAGE.

References

ab60300 has not yet been referenced specifically in any publications.

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