Overview

  • Product name

    Recombinant Human ITPK1 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMQTFLK GKRVGYWLSE KKIKKLNFQA FAELCRKRGM EVVQLNLSRP IEEQGPLDVI IHKLTDVILE ADQNDSQSLE LVHRFQEYID AHPETIVLDP LPAIRTLLDR SKSYELIRKI EAYMEDDRIC SPPFMELTSL CGDDTMRLLE KNGLTFPFIC KTRVAHGTNS HEMAIVFNQE GLNAIQPPCV VQNFINHNAV LYKVFVVGES YTVVQRPSLK NFSAGTSDRE SIFFNSHNVS KPESSSVLTE LDKIEGVFER PSDEVIRELS RALRQALGVS LFGIDIIINN QTGQHAVIDI NAFPGYEGVS EFFTDLLNHI ATVLQGQSTA MAATGDVALL RHSKLLAEPA GGLVGERTCS ASPGCCGSMM GQDAPWKAEA DAGGTAKLPH QRLGCNAGVS PSFQQHCVAS LATKASSQ
    • Molecular weight

      48 kDa including tags
    • Amino acids

      1 to 414
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab130021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Purity

    > 95 % SDS-PAGE.
    ab130021 was purified using conventional chromatography techniques.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.03% EDTA, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • 3
    • 4)P(3) 5/6-kinase
    • 4-trisphosphate 5/6-kinase
    • Inositol 1
    • Inositol 1 3 4 triphosphate 5/6 kinase
    • Inositol 1 3 4 trisphosphate 5/6 kinase
    • Inositol tetrakisphosphate 1 kinase
    • Inositol triphosphate 5/6 kinase
    • Inositol-tetrakisphosphate 1-kinase
    • Inositol-triphosphate 5/6-kinase
    • Ins(1
    • Ins(1 3 4)P(3) 5/6 kinase
    • ITPK 1
    • itpk1
    • ITPK1_HUMAN
    • ITRPK 1
    • ITRPK1
    see all
  • Function

    Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.
  • Tissue specificity

    Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.
  • Sequence similarities

    Belongs to the ITPK1 family.
    Contains 1 ATP-grasp domain.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab130021.

References

ab130021 has not yet been referenced specifically in any publications.

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