Recombinant Human IYD protein (denatured) (ab177648)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human IYD protein (denatured) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSDRSMEKKKGEPRTRAEARPWVDEDLKD SSDLHQAEEDADEWQESEENVEHIPFSHNHYPEKEMVKRSQEFYELLNKR RSVRFISNEQVPMEVIDNVIRTAGTAPSGAHTEPWTFVVVKDPDVKHKIR KIIEEEEEINYMKRMGHRWVTDLKKLRTNWIKEYLDTAPILILIFKQVHG FAANGKKKVH YYNE -
Predicted molecular weight
25 kDa including tags -
Amino acids
24 to 214 -
Tags
His tag N-Terminus -
Additional sequence information
Extracellular domain. NP_001158167.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab177648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 2.4% Urea
General Info
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Alternative names
- C6orf71
- DEHAL1
- dJ422F24.1
see all -
Function
Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine. -
Tissue specificity
Expressed at a high level in thyroid gland and at lower level in kidney and trachea. -
Involvement in disease
Defects in IYD are the cause of congenital hypothyroidism due to dyshormonogenesis type 4 (CHDH4) [MIM:274800]; also known as genetic defect in thyroid hormonogenesis type 4 or iodotyrosine dehalogenase deficiency or deiodinase deficiency. Patients with this defect present a phenotype of severe hypothyroidism, goiter, excessive levels of iodotyrosine in serum and urine, and variable mental deficits derived from unrecognized hypothyroidism. -
Sequence similarities
Belongs to the nitroreductase family. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human IYD protein (denatured) (ab177648)
15% SDS-PAGE showing ab177648 (3 µg) at approximately 25.1 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab177648 has not yet been referenced specifically in any publications.
