Recombinant Human JAB1 protein (ab131744)
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- Protocols
Description
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Product name
Recombinant Human JAB1 protein -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHH YFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPV EGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGI DVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGP SEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTL SSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKL AKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS -
Predicted molecular weight
62 kDa including tags -
Amino acids
1 to 334
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab131744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
ELISA
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
Note: Note: Reduced Glutathione
General Info
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Alternative names
- 38 kDa Mov34 homolog
- COP9 (constitutive photomorphogenic) homolog subunit 5
- COP9 constitutive photomorphogenic homolog subunit 5
see all -
Function
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. -
Sequence similarities
Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain. -
Domain
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human JAB1 protein (ab131744)12.5% SDS-PAGE ana;ysis of ab131744, stained with Coomassie Blue.
Datasheets and documents
References
ab131744 has not yet been referenced specifically in any publications.
