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Bioactive grade

Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)

  • Datasheet
Submit a review Q&A (2)References (1)

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Functional Studies - Recombinant Human Jagged1 protein (ab108575)
  • Flow Cytometry - Recombinant Human Jagged1 protein (ab108575)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level: < 0.100 Eu/µg
  • Active: Yes
  • Tags: Fc tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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ELISA
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Human Jagged1 ELISA Kit (ab267651)

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Description

  • Product name

    Recombinant human Jagged1 protein (Fc Chimera Active)
    See all Jagged1 proteins and peptides
  • Biological activity

    Induction of HES in NIH/3T3 cells.

    Inhibition of adipogenesis in NIH/3T3 cells.

  • Purity

    > 90 % SDS-PAGE.
    ab108575 is 0.2µm filtered
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    P78504
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      150 kDa including tags
    • Amino acids

      1 to 1067
    • Tags

      Fc tag C-Terminus
    • Additional sequence information

      Signal peptide and extracellular domain of human Jagged-1 (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG1.

Associated products

  • Related Products

    • Anti-Jagged1 antibody (ab7771)

Specifications

Our Abpromise guarantee covers the use of ab108575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: PBS

    0.2µm-filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AGS
    • AHD
    • AWS
    • CD 339
    • CD339
    • CD339 antigen
    • Headturner
    • hJ1
    • Htu
    • Jag 1
    • Jag1
    • JAG1_HUMAN
    • Jagged 1
    • Jagged1
    • Jagged1 (Alagille syndrome)
    • JAGL1
    • MGC104644
    • OTTHUMP00000030278
    • Protein jagged-1
    • Ser 1
    • Ser1
    • Serrate 1
    • Slalom
    see all
  • Function

    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • Tissue specificity

    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • Involvement in disease

    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • Sequence similarities

    Contains 1 DSL domain.
    Contains 15 EGF-like domains.
  • Developmental stage

    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P78504 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant Human Jagged1 protein (ab108575)
    Functional Studies - Recombinant Human Jagged1 protein (ab108575)
    Induction of Hes-1 with the treatment of recombinant Human Jagged1-Fc (ab108575).
    A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of ab108575 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

    M: Marker
    Lane 1: ab108575, 0 min
    Lane 2: ab108575, 10 min
    Lane 3: ab108575, 30 min
    Lane 4: ab108575, 1 hr
    Lane 5: ab108575, 2 hr
    Lane 6: ab108575, 4 hr
    Lane 7: ab108575, 8 hr
    Lane 8: ab108575, 24 hr
  • Flow Cytometry - Recombinant Human Jagged1 protein (ab108575)
    Flow Cytometry - Recombinant Human Jagged1 protein (ab108575)

    NIH/3T3 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin.

    When the cells reached confluence, adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0).

    The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (ab108575) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab108575? Please let us know so that we can cite the reference in this datasheet.

    ab108575 has been referenced in 1 publication.

    • Tang D  et al. Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin ß1 in Keratinocyte Migration. Sci Rep 7:43926 (2017). PubMed: 28266574

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    We talked this morning about the inaccurate protein concentration of the Jagged-1 protein. The Catalog number is AB108575-10UG, Lot number **, and the PO number is **Please see attached the commassie blue with BSA standard. Although I could see very clear band in 250ng BSA standard lane, I could not see any band in 250 ng Jagged-1 lane. This suggested that the Jagged-1 protein concentration is seriously off….. Could you please kindly take care of this problem for us? Thanks a lot!

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Thank you for contacting Abcam about ab108575.
    We checked the concentration of the protein that you received internally and everything was ok. We assume that something happened during the preparation/aliquotting of the protein. I have sent you another vial of ab108575, free of charge, to make up the fact that you did not receive the full 10ug in the first vial we sent you. Your new order number is ***.
    I do apologize for the inconvenience this has caused and if there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Question

    Dear Sir/madam: I plan to do the GSI treatment,but some literature said it should need recombinant human Jagged1-Fc, is the produce(Jagged1 protein (ab108575)) of your company meet for it?could you give me the protocol. Thanks

    Read More

    Abcam community

    Verified customer

    Asked on May 04 2012

    Answer

    Thank you for contacting us.


    While this product is indeedrecombinant Human Jagged1-Fc, we do not currently have a recommended protocol for yuse with this product. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests


    I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

    Read More

    Abcam Scientific Support

    Answered on May 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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