Key features and details
- Expression system: Baculovirus
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human JAK3 protein (Active)
See all JAK3 proteins and peptides
Specific Activity: 500 pmol/min/μg. Assay conditions: 40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr) substrate and 20 mM ATP. Reaction was done at 30°C for 45 min.
Purity>= 90 % SDS-PAGE.
Protein lengthProtein fragment
Actual molecular weight37 kDa including tags
Amino acids811 to 1124
TagsHis tag N-Terminus
Additional sequence informationNM_000215. N-terminal 10x His tag.
Our Abpromise guarantee covers the use of ab89982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C.
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 184.108.40.206
- JAK 3
- JAK L
FunctionTyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
Tissue specificityIn NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
Involvement in diseaseDefects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
modificationsTyrosine phosphorylated in response to IL-2 and IL-4.
Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab89982 has not yet been referenced specifically in any publications.