Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      70 kDa including tags
    • Amino acids

      1 to 424

Associated products

Specifications

Our Abpromise guarantee covers the use of ab119726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    ab119726 specific activity was determined to be 13 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity

    > 95 % SDS-PAGE.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 25% Glycerol, 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.003% DTT, 0.002% PMSF, 0.003% EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • c Jun kinase 2
    • C Jun N terminal kinase 2
    • c-Jun N-terminal kinase 2
    • JNK 55
    • JNK-55
    • JNK2
    • JNK2 alpha
    • JNK2 beta
    • JNK2A
    • JNK2alpha
    • JNK2B
    • JNK2BETA
    • Jun kinase
    • MAP kinase 9
    • MAPK 9
    • Mapk9
    • Mitogen activated protein kinase 9
    • Mitogen-activated protein kinase 9
    • MK09_HUMAN
    • P54a
    • p54aSAPK
    • PRKM9
    • Protein kinase, mitogen-activated, 9
    • SAPK
    • SAPK alpha
    • SAPK1a
    • Stress activated protein kinase 1a
    • Stress-activated protein kinase JNK2
    see all
  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
    JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro.
  • Information by UniProt

Images

  • ab119726 specific activity was determined to be 13 nmol/min/mg.
  • SDS Page analysis of ab119726

References

ab119726 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting Abcam with your questions pertaining to ab119726. I have been in contact with the laboratory about the questions that you have raised. The tag used in this preparation is GST. ab1198726 may be used in conjunction with cell study as it is highly likely that it provides cell permeability. It would depend on how you would transfer it into the cell. Currently there is no specific protocol for a functional study with this protein. Please do not hesitate to contact us if there is anything else that we might do to help you achieve you research goals.

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