Key features and details
- Expression system: Escherichia coli
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Josephin-2 protein (Active)
The specific activity of ab268693 was ≥28 nmol/min/mg in a deubiquitinase assay using recombinant human ubiquitin-based proluciferin as substrate.
Purity> 70 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMSQAPGAQPSPPTVYHERQRLELCAVHALNNVLQQQLFSQEAADEICKRL APDSRLNPHRSLLGTGNYDVNVIMAALQGLGLAAVWWDRRRPLSQLALPQ VLGLILNLPSPVSLGLLSLPLRRRHWVALRQVDGVYYNLDSKLRAPEALG DEDGVRAFLAAALAQGLCEVLLVVTKEVEEKGSWLRTD
Molecular weight information20 kDa by SDS-PAGE
Amino acids1 to 188
TagsHis tag N-Terminus
Additional sequence informationNM_138334
Our Abpromise guarantee covers the use of ab268693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- JOSD 2
FunctionCleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.
Sequence similaritiesContains 1 Josephin domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268693 has not yet been referenced specifically in any publications.