Recombinant Human KAT2B / PCAF protein (ab56275)
Key features and details
- Expression system: Escherichia coli
- Purity: > 70% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human KAT2B / PCAF protein
See all KAT2B / PCAF proteins and peptides -
Purity
> 70 % Densitometry. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
69 kDa -
Amino acids
431 to 832 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant fragment, corresponding to amino acids 431-end of Human PCAF.
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Specifications
Our Abpromise guarantee covers the use of ab56275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
PCAF is a binding protein that associates itself with p300/CBP and has no enzymatic activity. However storage at -80C and in glycerol is considered to prevent denaturation and to ensure that its binding conformation is conserved.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride
General Info
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Alternative names
- CAF
- CREBBP associated factor
- GCN5
see all -
Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. -
Tissue specificity
Ubiquitously expressed but most abundant in heart and skeletal muscle. -
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
Domain
The bromodomain mediates binding to HIV-1 Tat. -
Cellular localization
Nucleus. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab56275 has not yet been referenced specifically in any publications.