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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation
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Recombinant Human KDM1/LSD1 protein (ab125649)

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SDS-PAGE - Recombinant Human KDM1/LSD1 protein (ab125649)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 95% Densitometry
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human KDM1/LSD1 protein
      See all KDM1/LSD1 proteins and peptides
    • Purity

      > 95 % Densitometry.

    • Expression system

      Baculovirus infected Sf9 cells
    • Accession

      O60341
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Predicted molecular weight

        100 kDa including tags
      • Amino acids

        172 to 852

    Associated products

    • Related Products

      • Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)

    Specifications

    Our Abpromise guarantee covers the use of ab125649 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • Amine oxidase (flavin containing) domain 2
      • Amine oxidase, flavin containing, 2
      • AOF2
      • BHC110
      • BRAF35 HDAC complex protein BHC110
      • BRAF35-HDAC complex protein BHC110
      • BRAF35/HDAC complex, 110-kD subunit
      • CPRF
      • EC1
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit
      • Flavin-containing amine oxidase domain-containing protein 2
      • KDM 1
      • KDM1
      • Kdm1a
      • KDM1A_HUMAN
      • KIAA0601
      • LSD 1
      • LSD1
      • Lysine (K) specific demethylase 1
      • Lysine (K) specific demethylase 1A
      • Lysine demethylase 1A
      • Lysine specific histone demethylase 1
      • Lysine specific histone demethylase 1A
      • Lysine-specific demethylase 1
      • Lysine-specific demethylase 1A
      • Lysine-specific histone demethylase 1A
      see all
    • Function

      Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the flavin monoamine oxidase family.
      Contains 1 SWIRM domain.
    • Domain

      The SWIRM domain may act as an anchor site for a histone tail.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O60341 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human KDM1/LSD1 protein (ab125649)
      SDS-PAGE - Recombinant Human KDM1/LSD1 protein (ab125649)
      SDS-PAGE analysis of ab125649.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab125649? Please let us know so that we can cite the reference in this datasheet.

    ab125649 has not yet been referenced specifically in any publications.

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