Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 82% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human KDM4C / GASC1 / JMJD2C protein (Tagged)
See all KDM4C / GASC1 / JMJD2C proteins and peptides
Specific Activity: 0.17 pmole/min/μg.
Assay Conditions: 10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab198094 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
Purity>= 82 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceEVAEVESPLNPSCKIMTFRPSMEEFREFNKYLAYMESKGAHRAGLAKVIP PKEWKPRQCYDDIDNLLIPAPIQQMVTGQSGLFTQYNIQKKAMTVKEFRQ LANSGKYCTPRYLDYEDLERKYWKNLTFVAPIYGADINGSIYDEGVDEWN IARLNTVLDVVEEECGISIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINY LHFGEPKSWYAIPPEHGKRLERLAQGFFPSSSQGCDAFLRHKMTLISPSV LKKYGIPFDKITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATVRWIDY GKVAKLCTCRKDMVKISMDIFVRKFQPDRYQLWKQGKDIYTIDHTKPTPA STPEVKAWLQRRRKVRKASRS
Predicted molecular weight69 kDa including tags
Amino acids2 to 372
TagsGST tag N-Terminus
Additional sequence informationGenBank Accession No. BC143571
Our Abpromise guarantee covers the use of ab198094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 20% Glycerol, 0.49% Glutathione
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- GASC 1 protein
- GASC-1 protein
FunctionHistone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
Tissue specificityOverexpressed in several esophageal squamous cell carcinomas (ESCs).
Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
DomainThe 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198094 has not yet been referenced specifically in any publications.