Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human KDM4D / JMJD2D protein
Specific Activity: 0.017 pmole/min/μg
Assay Conditions: 10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196398 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
Purity> 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceETMKSKANCAQNPNCNIMIFHPTKEEFNDFDKYIAYMESQGAHRAGLAKI IPPKEWKARETYDNISEILIATPLQQVASGRAGVFTQYHKKKKAMTVGEY RHLANSKKYQTPPHQNFEDLERKYWKNRIYNSPIYGADISGSLFDENTKQ WNLGHLGTIQDLLEKECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSI NYLHLGEPKTWYVVPPEHGQRLERLARELFPGSSRGCGAFLRHKVALISP TVLKENGIPFNRITQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWI DYGKMASQCSCGEARVTFSMDAFVRILQPERYDLWKRGQDRAVVDHMEPR VPA
Predicted molecular weight67 kDa including tags
Amino acids2 to 354
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab196398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.12% Glutathione, 20% Glycerol, 0.05% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- JmjC domain-containing histone demethylation protein 3D
FunctionHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196398 has not yet been referenced specifically in any publications.