Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human KDM5A / Jarid1A / RBBP2 protein
See all KDM5A / Jarid1A / RBBP2 proteins and peptides
Specific Activity: 0.027 pmole/min/μg
Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab198108 (80 – 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Purity>= 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICK IRPPKDWQPPFACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWEL QGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWSKVGSRLGYLP GKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTD TQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVG LAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCG RGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPRE AFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSS IEEDVIVEYGADISSKDFGSGFPVKDGRRKILPEEEEYALSGWNLNNMPV LEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEP KTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGV PVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVN HYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLR ESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCL YHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEAL SANFNHKKDLIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCASVAQL LLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVISQARQVKNLL DDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQ ARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLT VSERWEEKAKVCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQK AREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAA RAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRR
Predicted molecular weight125 kDa including tags
Amino acids1 to 1090
TagsDDDDK tag C-Terminus
Additional sequence informationNM_001042603.
Our Abpromise guarantee covers the use of ab198108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
80 µg/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Histone demethylase JARID1A
- Jumonji/ARID domain containing protein 1A
FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.
Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
DomainThe GSGFP motif is required for the interaction with SUZ12.
Cellular localizationNucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198108 has not yet been referenced specifically in any publications.