Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human KDM5C / Jarid1C / SMCX protein
See all KDM5C / Jarid1C / SMCX proteins and peptides
Specific activity of 0.078 pmol/min/µg.
Purity>= 85 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceEPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPAD WQPPFAVEV DNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSS LKIPNVERRILDLYSLSKIVV EEGGYEAICKDRRWARVAQRLNYPPGK NIGSLLRSHYERIVYPYEMYQSGANLVQCNTRP FDNEEKDKEYKPHSI PLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYG AGP KMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLE SKEELSH SPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLL CDGCDDNYHIFCLLPPLPE IPKGVWRCPKCVMAECKRPPEAFGFEQAT REYTLQSFGEMADSFKADYFNMPVHMVPTEL VEKEFWRLVNSIEEDVT VEYGADIHSKEFGSGFPVSDSKRHLTPEEEEYATSGWNLNVMP VLEQS VLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWY GVPSL AAEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPV VRTNQCAGEFVITFPRA YHSGFNQGYNFAEAVNFCTADWLPAGRQCIE HYRRLRRYCVFSHEELICKMAACPEKLDL NLAAAVHKEMFIMVQEERR LRKALLEKGITEAEREAFELLPDDERQCIKCKTTCFLSALA CYDCPDG LVCLSHINDLCKCSSSRQYLRYRYTLDELPAMLHKLKVRAESFDTWANKV RVA LEVEDGRKRSLEELRALESEARERRFPNSELLQQLKNCLSEAEAC VSRALGLVSGQEAGP HRVAGLQMTLTELRAFLDQMNNLPCAMHQIGDV KGVLEQVEAYQAEAREALASLPSSPGL LQSLLERGRQLGVEVPEAQQL QRQVEQARWLDEVKRTLAPSARRGTLAVMRGLLVAGASV APSPAVDKA QAELQELLTIAERWEEKAHLCLEARQKHPPATLEAIIREAENIPVHLPNI Q ALKEALAKARAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEE LRQLELQVLTAHS WREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKR SRWMEKELGLYKSDTELLGLSAQDL RDPGSVIVAFKEGEQKEKEGILQ LRRTNSAKPSPLASSSTASSTTSICVCGQVLAGAGAL QCDLCQDWFHG RCVSVPRLLSSPRPNPTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILA LLVALQRLPVRLPEGEALQCLTERAISWQGRARQALASEDVTALLGRLA ELRQRLQAEPR PEEPPNYPAAPASDPLREGSGKDMPKVQGLLENGDSV TSPEKVAPEEGSGKRDLELLSSL LPQLTGPVLELPEATRAPLEELMME GDLLEVTLDENHSIWQLLQAGQPPDLERIRTLLEL EKAERHGSRARGR ALERRRRRKVDRGGEGDDPAREELEPKRVRSSGPEAEEVQEEEELEE E TGGEGPPAPIPTTGSPSTQENQNGLEPAEGTTSGPSAPFSTLTPRLHLPC PQQPPQQQL
Predicted molecular weight181 kDa including tags
Amino acids2 to 1560
TagsHis tag N-Terminus , DDDDK tag N-Terminus
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab196392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 64% Sodium chloride, 0.02% Potassium chloride, 0.462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
Also contains 80 µg/ml DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Histone demethylase JARID1C
FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Tissue specificityExpressed in all tissues examined. Highest levels found in brain and skeletal muscle.
Involvement in diseaseDefects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
Sequence similaritiesBelongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
DomainThe first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
- Information by UniProt
10 µL reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196392 (50 – 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
10% SDS PAGE with Coomassie staining: 6 µg ab196392.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196392 has not yet been referenced specifically in any publications.