Description

  • Product name

    Recombinant human KDM6B / JMJD3 protein
  • Biological activity

    Specific activity: 0.022 pmole/min/μg.

    10µl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 150 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD3 (100 – 500 ng) added to the wells. Add antibody against demethylated K27 peptide.

  • Purity

    >= 50 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      KAKPPTAP APPSAPAPSA QPTPPSASVP GKKAREEAPG PPGVSRADML KLRSLSEGPP KELKIRLIKV ESGDKETFIA SEVEERRLRM ADLTISHCAA DVVRASRNAK VKGKFRESYL SPAQSVKPKI NTEEKLPREK LNPPTPSIYL ESKRDAFSPV LLQFCTDPRN PITVIRGLAG SLRLNLGLFS TKTLVEASGE HTVEVRTQVQ QPSDENWDLT GTRQIWPCES SRSHTTIAKY AQYQASSFQE SLQEEKESED EESEEPDSTT GTPPSSAPDP KNHHIIKFGT NIDLSDAKRW KPQLQELLKL PAFMRVTSTG NMLSHVGHTI LGMNTVQLYM KVPGSRTPGH QENNNFCSVN INIGPGDCEW FAVHEHYWET ISAFCDRHGV DYLTGSWWPI LDDLYASNIP VYRFVQRPGD LVWINAGTVH WVQATGWCNN IAWNVGPLTA YQYQLALERY EWNEVKNVKS IVPMIHVSWN VARTVKISDP DLFKMIKFCL LQSMKHCQVQ RESLVRAGKK IAYQGRVKDE PAYYCNECDV EVFNILFVTS ENGSRNTYLV HCEGCARRRS AGLQGVVVLE QYRTEELAQA YDAFTLVRAR RARGQRRRAL GQAAGTGFGS PAAPFPEPPP AFSPQAPAST SR
    • Predicted molecular weight

      71 kDa including tags
    • Amino acids

      1043 to 1643
    • Tags

      DDDDK tag C-Terminus
    • Additional sequence information

      NM_001080424.

Specifications

Our Abpromise guarantee covers the use of ab196420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol, 0.05% DTT

    Also contains 90 µg/ml DDDDK peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Histone demethylase JMJD3
    • JmjC domain containing protein 3
    • JmjC domain-containing protein 3
    • Jumonji D3
    • Jumonji domain containing 3
    • Jumonji domain containing 3 histone lysine demethylase
    • Jumonji domain containing protein 3
    • Jumonji domain-containing protein 3
    • Kdm6b
    • KDM6B_HUMAN
    • KIAA0346
    • Lysine demethylase 6B
    • Lysine K specific demethylase 6B
    • Lysine specific demethylase 6B
    • Lysine-specific demethylase 6B
    see all
  • Function

    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • Sequence similarities

    Belongs to the UTX family.
    Contains 1 JmjC domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE of 4μg ab196420 (lane 1). Protein marker (lane 2).
    10% gel, coomassie staining.

  • Activity assay for ab196420. 

References

ab196420 has not yet been referenced specifically in any publications.

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