Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      22 kDa

Specifications

Our Abpromise guarantee covers the use of ab9601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).

  • Applications

    Cell differentiation

    Functional Studies

    SDS-PAGE

  • Form
    Lyophilised
  • Additional notes
    The ED50, determined by the dose-dependent stimulation of of thymidine uptake by KGF-responsive BaF3 cells, is < 10.0 ng/ml, corresponding to a specific activity of > 1.0 x 105 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    PBS, pH 7.0, no preservative, sterile filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • FGF 7
    • FGF-7
    • Fgf7
    • FGF7_HUMAN
    • Fibroblast growth factor 7
    • HBGF 7
    • HBGF-7
    • HBGF7
    • Heparin binding growth factor 7
    • Heparin-binding growth factor 7
    • Keratinocyte growth factor
    • KGF
    see all
  • Function
    Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
  • Tissue specificity
    Epithelial cell.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • Definitive endoderm derived from culturing human embryonic stem cells (HUES4) with Inhibin beta a (ab50051) and Wnt3a (ab81484) was further differentiated to gut tube endoderm as described in Kroon et al, Nat Biotechnol. (2008). KGF (ab9601) was added to the media to direct differentiation towards gut tube endoderm. This image was taken 3 days after addition of KGF, where the gut tube endoderm marker TCF2 (ab59118 in green) is present. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

References

ab9601 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your interesting enquiry regaring ab9601. The peptide/proteinproduct we supply are often used as controls for western blotting. However, there are quite a few references you may be interested in which I have listed below. With regards to protein degradation, freeze thaw cycles should be kept to a minimum. I would recommend reconstitution in PBS, then aliquot and store at -20oC. Working aliquots can be stored at +4oC short term. Extreme pH and denaturing reagents will also degrade the protein. I hope this information is helpful. Please do not hesitate to contact me again should you have any more questions. REFERENCES: Farley SM et al. Fas Ligand Elicits a Caspase-Independent Proinflammatory Response in Human Keratinocytes: Implications for Dermatitis. Journal of Investigative Dermatology 7/20/2006 Pub med ref 16858424 Zhang X et al Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family. J Biol Chem.;281(23):15694-700 6/9/2006 Pubmed ref 16597617 McDowell LM et al Inhibition or activation of apert syndrome FGFR2 (S252W) signaling by specific glycosaminoglycans. J Biol Chem;281(11):6924-30 3/17/2006 Pubmed ref 16373332 Newton DA et al. Hemoglobin is expressed by alveolar epithelial cells. J Biol Chem;281(9):5668-76 3/3/2006 Pubmed ref 16407281 Chen C et al. ERM is required for transcriptional control of the spermatogonial stem cell niche. Nature; 436(7053):1030-4 8/18/2005 Pubmed ref 16107850 Tamaru N et al. Estrogen receptor-associated expression of keratinocyte growth factor and its possible role in the inhibition of apoptosis in human breast cancer Laboratory Investigation; 84(11):1460-71 11/1/2004 Pubmed ref 15311216 Naoe Tamaru et al. Estrogen receptor-associated expression of keratinocyte growth factor and its possible role in the inhibition of apoptosis in human breast cancer Lab. Ivest, Vol:8/16/2004 15311216 Gobran LI et al. Pulmonary surfactant secretion in briefly cultured mouse type II cells. Am J Physiol Lung Cell Mol Physiol.; 286(2):L331-6 2/1/2004. Pubmed ref 14565945 James P. Bridges et al. Expression of a human surfactant protein C mutation associated with interstitial lung disease disrupts lung development in transgenic mice J. Bio. Chem. Vol: 278 (52) pp. 52739-52746 12/26/2003. Pubmed ref 14525980 Murakami S et al. Up-regulation of the chondrogenic Sox9 gene by fibroblast growth factors is mediated by the mitogen-activated protein kinase pathway Proc. Natl. Acad Sci. USA. Volume 97. Issue 3. Pp.1113-1118. 2/1/2000 Pubmed ref 10655493 Maatta A et al. Extracellular Matrix-dependent Activation of Syndecan-1 Expression in Keratinocyte Growth Factor-treated Keratinocytes* J.Biol Chem, Volume 274, Issue 14, pp.9891-9898 4/2/1999 Pubmed ref 10092681 Jaakkola P et al. Wound reepithelialization activates a growth factor-responsive enhancer in migrating keratinocytes The FASEB Journal.12: pp.959-969 1/1/1998 Pubmed ref 9707168

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up