Recombinant human KIAA1549 + BRAF protein (ab204200)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
-
Product name
Recombinant human KIAA1549 + BRAF protein
See all KIAA1549 + BRAF proteins and peptides -
Biological activity
The specific activity of ab204200 was determined to be 550 nmol/min/mg.
-
Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Amino Acid Sequence 1
-
Species
Human -
Sequence
DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSS SSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQ WCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSN NIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDK NPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSK VRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASE PSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH -
Predicted molecular weight
125 kDa including tags -
Amino acids
381 to 766 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_004333.
-
Amino Acid Sequence 2
-
Species
Human -
Sequence
YWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDIL IIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADST VSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHV DRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQ TALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQ MQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRL ITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFP ASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLP ANSTPSQEERRATQWGSFYSPAQTANNPCS -
Amino acids
1320 to 1749 -
Additional sequence information
NM_001164665.
-
Specifications
Our Abpromise guarantee covers the use of ab204200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- BRAF1
- p94
- Proto-oncogene B-Raf
see all -
Relevance
BRAF: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. -
Cellular localization
BRAF: Cell Membrane, Cytoplasmic and Nuclear; KIAA1549: Membrane; Multi-pass membrane protein
Images
-
Functional Studies - Recombinant human KIAA1549 + BRAF protein (ab204200)
Kinase assay showing the specific activity of ab204200 as 550 nmol/min/mg.
-
SDS-PAGE - Recombinant human KIAA1549 + BRAF protein (ab204200)SDS-PAGE analysis of ab204200. Predicted Mw 125KDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab204200 has not yet been referenced specifically in any publications.
