Recombinant human KIAA1549 + BRAF protein (ab204200)
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Description
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Product name
Recombinant human KIAA1549 + BRAF protein
See all KIAA1549 + BRAF proteins and peptides -
Biological activity
The specific activity of ab204200 was determined to be 550 nmol/min/mg.
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Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSS SSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQ WCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSN NIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDK NPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSK VRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASE PSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH -
Predicted molecular weight
125 kDa including tags -
Amino acids
381 to 766 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_004333.
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Amino Acid Sequence 2
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Species
Human -
Sequence
YWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDIL IIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADST VSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHV DRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQ TALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQ MQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRL ITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFP ASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLP ANSTPSQEERRATQWGSFYSPAQTANNPCS -
Amino acids
1320 to 1749 -
Additional sequence information
NM_001164665.
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Specifications
Our Abpromise guarantee covers the use of ab204200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- BRAF1
- p94
- Proto-oncogene B-Raf
see all -
Relevance
BRAF: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. -
Cellular localization
BRAF: Cell Membrane, Cytoplasmic and Nuclear; KIAA1549: Membrane; Multi-pass membrane protein
Images
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Functional Studies - Recombinant human KIAA1549 + BRAF protein (ab204200)Kinase assay showing the specific activity of ab204200 as 550 nmol/min/mg.
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SDS-PAGE - Recombinant human KIAA1549 + BRAF protein (ab204200)SDS-PAGE analysis of ab204200. Predicted Mw 125KDa.
Datasheets and documents
References
ab204200 has not yet been referenced specifically in any publications.
