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Recombinant Human Kininogen 1 protein (His tag) (ab220569)

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SDS-PAGE - Recombinant Human Kininogen 1 protein (His tag) (ab220569)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 92% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human Kininogen 1 protein (His tag)
      See all Kininogen 1 proteins and peptides
    • Purity

      > 92 % SDS-PAGE.
      Lyophilized from 0.22 µm filtered solution.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      P01042-2
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGS DTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTK FSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFN NNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDC KSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCP RDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYF IDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTV NCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGA EPASEREVS
      • Predicted molecular weight

        47 kDa including tags
      • Amino acids

        19 to 427
      • Tags

        His tag C-Terminus
      • Additional sequence information

        NP_000884. Predicted N-terminus: Gln 19.

    Associated products

    • Related Products

      • Anti-Kininogen 1 antibody (ab175386)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab220569 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: 5% Trehalose, 0.58% Sodium chloride, 0.8% Sodium acetate

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

    General Info

    • Alternative names

      • Alpha-2-thiol proteinase inhibitor
      • BDK
      • BK
      • Bradykinin
      • Bradykinin included
      • Fitzgerald factor
      • FLAUJEAC FACTOR
      • High molecular weight kininogen
      • HMWK
      • Ile-Ser-Bradykinin
      • Kallidin I
      • Kallidin II
      • KNG
      • KNG1
      • KNG1_HUMAN
      • Low molecular weight growth-promoting factor
      • WILLIAMS FACTOR
      • Williams-Fitzgerald-Flaujeac factor
      see all
    • Function

      (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
    • Tissue specificity

      Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
    • Involvement in disease

      Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
    • Sequence similarities

      Contains 3 cystatin domains.
    • Post-translational
      modifications

      Bradykinin is released from kininogen by plasma kallikrein.
      Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
      Phosphorylation sites are present in the extracelllular medium.
      N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    • Cellular localization

      Secreted > extracellular space.
    • Target information above from: UniProt accession P01042 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Kininogen 1 protein (His tag) (ab220569)
      SDS-PAGE - Recombinant Human Kininogen 1 protein (His tag) (ab220569)

      SDS-PAGE (DTT reduced) analysis of ab220569 followed by staining overnight with Coomassie Blue.

      The protein migrates as 50-68 kDa under reducing condition (SDS-PAGE) due to glycosylation.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab220569? Please let us know so that we can cite the reference in this datasheet.

    ab220569 has not yet been referenced specifically in any publications.

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