Name: Recombinant human KLF4 protein
SKU: ab169841

Product name

Recombinant human KLF4 protein
See all KLF4 proteins and peptides
Protein length

Full length protein

Nature

Recombinant
Source

Escherichia coli
Amino Acid Sequence
- Accession
  
  O43474-1
- Species
  
  Human
- Sequence
  
  MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSH MKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFN DLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSF TYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFV AELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISV SKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRP AAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPS FLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDY AGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKH TGHRPFQCQKCDRAFSRSDHLALHMKRHFESGGGGSPGRRRRRRRRRRR
- Molecular weight
  
  53 kDa including tags
- Amino acids
  
  1 to 479

Our Abpromise guarantee covers the use of ab169841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Biological activity

The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.
Applications

Functional Studies

SDS-PAGE
Purity

> 75 % SDS-PAGE.
Form

Liquid
Additional notes

The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.
Concentration information loading...

Stability and Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

pH: 7.50
Constituents: Potassium chloride, 0.24% Tris, EDTA, Glycerol, Sodium chloride
Note: Also contains DTT and Arginine.

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Alternative names
- Endothelial Kruppel like zinc finger protein
- Epithelial zinc finger protein EZF
- EZF
- GKLF
- gut Kruppel-like factor
- Gut-enriched krueppel-like factor
- KLF
- KLF4
- KLF4_HUMAN
- Krueppel-like factor 4
- Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor)
- Kruppel like factor 4 (gut)
see all
Function

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Domain

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Cellular localization

Nucleus.
Target information above from: UniProt accession O43474 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

Datasheet

ab169841 has not yet been referenced specifically in any publications.

Publishing research using ab169841? Please let us know so that we can cite the reference in this datasheet.

Location selector

Your location is currently set to:

Custom solutions & partnerships

Recombinant human KLF4 protein (ab169841)

Overview

Description

Specifications

Preparation and Storage

General Info

Datasheets and documents

References

Customer reviews and Q&As