Recombinant human KLF4 protein (ab169841)

Submit a review Submit a question

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 75% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human KLF4 protein
    See all KLF4 proteins and peptides

  • Biological activity

    The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.

  • Purity

    > 75 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    O43474-1

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSH MKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFN DLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSF TYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFV AELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISV SKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRP AAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPS FLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDY AGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKH TGHRPFQCQKCDRAFSRSDHLALHMKRHFESGGGGSPGRRRRRRRRRRR

    • Predicted molecular weight

      53 kDa including tags

    • Amino acids

      1 to 479

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab169841 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 7.50
      Constituents: Potassium chloride, 0.24% Tris, EDTA, Glycerol, Sodium chloride

      Also contains DTT and Arginine.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Endothelial Kruppel like zinc finger protein
      • Epithelial zinc finger protein EZF
      • EZF
      • GKLF
      • gut Kruppel-like factor
      • Gut-enriched krueppel-like factor
      • KLF
      • KLF4
      • KLF4_HUMAN
      • Krueppel-like factor 4
      • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor)
      • Kruppel like factor 4 (gut)
      see all

    • Function

      Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.

    • Domain

      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab169841? Please let us know so that we can cite the reference in this datasheet.

    ab169841 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab169841.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com