Recombinant Human KLHL12 protein (ab169886)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: T7 tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human KLHL12 protein -
Purity
> 90 % SDS-PAGE.
The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGEFMGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDV TLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKGKPYVDIQGLTASTME ILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNC LGIRDFAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKC DEIQVDSEEPVFEAVINWVKHAKKEREESLPNLLQYVRMPLLTPRYITDV IDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLV VGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIG GYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSG GFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGVIYCLGGYD GLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTA HLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLS SIECYDPIIDSWEVVTSMGTQRCDAGVCVLREK -
Predicted molecular weight
63 kDa -
Amino acids
1 to 568 -
Tags
T7 tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab169886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
General Info
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Alternative names
- C3IP1
- CUL3 interacting protein 1
- CUL3-interacting protein 1
see all -
Function
Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3. -
Tissue specificity
Highly expressed in testis and at lower levels in the submandibular salivary gland. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
Domain
The BTB domain is required for interaction with CUL3. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab169886 has not yet been referenced specifically in any publications.