Recombinant Human KMT4 / Dot1L protein (ab176960)

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SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)
  • SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: proprietary tag N-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human KMT4 / Dot1L protein
    See all KMT4 / Dot1L proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    Q8TEK3

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      GEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDL KLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTR PSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEI KMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDRE FRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDH QLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKG SVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKS NAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNK KGRKMAGRKRGRPKK

    • Predicted molecular weight

      74 kDa including tags

    • Amino acids

      2 to 416

    • Tags

      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab176960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.242% Tris, 1.17% Sodium chloride, 0.03% EDTA, 0.02% DTT

General Info

  • Alternative names

    • Disrupter of telomere silencing protein 1
    • DOT 1
    • DOT1
    • DOT1 like
    • DOT1 like histone H3 methyltransferase
    • DOT1 like histone H3 methyltransferase (S. cerevisiae)
    • DOT1 like histone H3K79 methyltransferase
    • DOT1 like protein
    • DOT1, S. cerevisiae, homolog of
    • DOT1-like protein
    • DOT1L
    • DOT1L_HUMAN
    • EC 2.1.1.43
    • H3 K79 HMTase
    • H3 lysine-79 specific
    • H3-K79-HMTase
    • Histone H3 K79 methyltransferase
    • Histone H3 methyltransferase
    • Histone H3 methyltransferase DOT1-like
    • Histone H3-K79 methyltransferase
    • Histone lysine N methyltransferase H3 lysine 79 specific
    • Histone methyltransferase DOT1L
    • Histone-lysine N-methyltransferase
    • KIAA1814
    • KMT4
    • Lysine N methyltransferase 4
    • Lysine N-methyltransferase 4
    see all

  • Function

    Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

  • Sequence similarities

    Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
    Contains 1 DOT1 domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)
    SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)

    SDS-PAGE analysis of ab176960
    Lane 1: Markers:
    Lane 2: ab176960 (1 μg)
    Lane 3: ab176960 (2 μg)
    Lane 4: ab176960 (4 μg)

  • SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)
    SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)

    Fluorograph of nucleosomes separated on SDS-PAGE following reaction with [3H]-KMT4 / Dot1L.

    Lane 1: ab176960 (0.5 μM)
    Lane 2: ab176960 (1 μM)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab176960? Please let us know so that we can cite the reference in this datasheet.

ab176960 has not yet been referenced specifically in any publications.

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