Recombinant Human KMT4 / Dot1L protein (ab176960)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q8TEK3
    • Species
      Human
    • Sequence
      GEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDL KLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTR PSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEI KMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDRE FRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDH QLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKG SVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKS NAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNK KGRKMAGRKRGRPKK
    • Molecular weight
      74 kDa including tags
    • Amino acids
      2 to 416
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab176960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • Disrupter of telomere silencing protein 1
    • DOT 1
    • DOT1
    • DOT1 like
    • DOT1 like histone H3 methyltransferase
    • DOT1 like histone H3 methyltransferase (S. cerevisiae)
    • DOT1 like histone H3K79 methyltransferase
    • DOT1 like protein
    • DOT1, S. cerevisiae, homolog of
    • DOT1-like protein
    • DOT1L
    • DOT1L_HUMAN
    • EC 2.1.1.43
    • H3 K79 HMTase
    • H3 lysine-79 specific
    • H3-K79-HMTase
    • Histone H3 K79 methyltransferase
    • Histone H3 methyltransferase
    • Histone H3 methyltransferase DOT1-like
    • Histone H3-K79 methyltransferase
    • Histone lysine N methyltransferase H3 lysine 79 specific
    • Histone methyltransferase DOT1L
    • Histone-lysine N-methyltransferase
    • KIAA1814
    • KMT4
    • Lysine N methyltransferase 4
    • Lysine N-methyltransferase 4
    see all
  • Function
    Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
  • Sequence similarities
    Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
    Contains 1 DOT1 domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Human KMT4 / Dot1L protein fragment (ab176960)
    SDS-PAGE - Human KMT4 / Dot1L protein fragment (ab176960)

    SDS-PAGE analysis of ab176960
    Lane 1: Markers:
    Lane 2: ab176960 (1 μg)
    Lane 3: ab176960 (2 μg)
    Lane 4: ab176960 (4 μg)

  • SDS-PAGE - Human KMT4 / Dot1L protein fragment (ab176960)
    SDS-PAGE - Human KMT4 / Dot1L protein fragment (ab176960)

    Fluorograph of nucleosomes separated on SDS-PAGE following reaction with [3H]-KMT4 / Dot1L.

    Lane 1: ab176960 (0.5 μM)
    Lane 2: ab176960 (1 μM)

References

ab176960 has not yet been referenced specifically in any publications.

Publishing research using ab176960? Please let us know so that we can cite the reference in this datasheet.

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