Recombinant Human KMT4 / Dot1L protein (ab176960)

Product name

Recombinant Human KMT4 / Dot1L protein
See all KMT4 / Dot1L proteins and peptides
Protein length

Protein fragment

Our Abpromise guarantee covers the use of ab176960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Stability and Storage

Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

Information available upon request.

Alternative names
- Disrupter of telomere silencing protein 1
- DOT 1
- DOT1
- DOT1 like
- DOT1 like histone H3 methyltransferase
- DOT1 like histone H3 methyltransferase (S. cerevisiae)
- DOT1 like histone H3K79 methyltransferase
- DOT1 like protein
- DOT1, S. cerevisiae, homolog of
- DOT1-like protein
- DOT1L
- DOT1L_HUMAN
- EC 2.1.1.43
- H3 K79 HMTase
- H3 lysine-79 specific
- H3-K79-HMTase
- Histone H3 K79 methyltransferase
- Histone H3 methyltransferase
- Histone H3 methyltransferase DOT1-like
- Histone H3-K79 methyltransferase
- Histone lysine N methyltransferase H3 lysine 79 specific
- Histone methyltransferase DOT1L
- Histone-lysine N-methyltransferase
- KIAA1814
- KMT4
- Lysine N methyltransferase 4
- Lysine N-methyltransferase 4
see all
Function

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
Contains 1 DOT1 domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q8TEK3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)

SDS-PAGE analysis of ab176960
Lane 1: Markers:
Lane 2: ab176960 (1 μg)
Lane 3: ab176960 (2 μg)
Lane 4: ab176960 (4 μg)
SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (ab176960)

Fluorograph of nucleosomes separated on SDS-PAGE following reaction with [³H]-KMT4 / Dot1L.
Lane 1: ab176960 (0.5 μM)
Lane 2: ab176960 (1 μM)

ab176960 has not yet been referenced specifically in any publications.

Publishing research using ab176960? Please let us know so that we can cite the reference in this datasheet.

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