The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
50 µl reaction mix (50 mM Tris, pH 8.8, 1 mM EDTA, 1 mM DTT, 40 µM S-adenosylhomocysteine, and 0-6 µg ab196432) is added to the wells coated with the substrate. Incubate for 2 hr. Add antibody against methylated residue of histone H4, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminsecent substrates and read luminescence.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
H4 K20 HMTase
H4 K20 specific histone methyltransferase
Histone H4 K20 methyltransferase
Histone lysine N methyltransferase H4 lysine 20 specific
Histone-lysine N-methyltransferase SETD8
Lysine N-methyltransferase 5A
N-lysine methyltransferase SETD8
Pr SET 7
PR/SET domain containing protein 07
PR/SET domain containing protein 8
PR/SET domain-containing protein 07
SET domain containing (lysine methyltransferase) 8
SET domain containing 8
SET domain containing lysine methyltransferase 8
SET domain containing protein 8
SET domain-containing protein 8
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Contains 1 SET domain.
Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.