Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human KSR1 (mutated A637F) protein (Active)
See all KSR1 proteins and peptides
The specific activity of ab268715 was determined to be 3.2 nmol/min/mg in a kinase assay using CREBtide synthetic peptide substrate.
Purity> 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMVRRDIGLSV THRFSTKSWL SQVCHVCQKS MIFGVKCKHC RLKCHNKCTK EAPACRISFL PLTRLRRTES VPSDINNPVD RAAEPHFGTL PKALTKKEHP PAMNHLDSSS NPSSTTSSTP SSPAPFPTSS NPSSATTPPN PSPGQRDSRF NFPAAYFIHH RQQFIFPVPS AGHCWKCLLI AESLKENAFN ISAFAHAAPL PEAADGTRLD DQPKADVLEA HEAEAEEPEA GKSEAEDDED EVDDLPSSRR PWRGPISRKA SQTSVYLQEW DIPFEQVELG EPIGQGRWGR VHRGRWHGEV FIRLLEMDGH NQDHLKLFKK EVMNYRQTRH ENVVLFMGAC MNPPHLAIIT SFCKGRTLHS FVRDPKTSLD INKTRQIAQE IIKGMGYLHA KGIVHKDLKS KNVFYDNGKV VITDFGLFGI SGVVREGRRE NQLKLSHDWL CYLAPEIVRE MTPGKDEDQL PFSKAADVYA FGTVWYELQA RDWPLKNQAA EASIWQIGSG EGMKRVLTSV SLGKEVSEIL SACWAFDLQE RPSFSLLMDM LEKLPKLNRR LSHPGHFWKS ADINSSKVVP RFERFGLGVL ESSNPKM
Molecular weight informationSDS-PAGE molecular weight: ~105kDa
Amino acids337 to 923
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab268715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- BKSR 1
FunctionLocation-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
modificationsPhosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
Cellular localizationCytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268715 has not yet been referenced specifically in any publications.