Recombinant human KSR1 protein (ab190396)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human KSR1 protein
See all KSR1 proteins and peptides -
Biological activity
The specific activity of ab190396 was determined to be 8 nmol/min/mg.
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Purity
> 85 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
KEHPPAMNHL DSSSNPSSTT SSTPSSPAPF PTSSNPSSAT TPPNPSPGQR DSRFNFPAAY FIHHRQQFIF PVPSAGHCWK CLLIAESLKE NAFNISAFAH AAPLPEAADG TRLDDQPKAD VLEAHEAEAE EPEAGKSEAE DDEDEVDDLP SSRRPWRGPI SRKASQTSVY LQEWDIPFEQ VELGEPIGQG RWGRVHRGRW HGEVAIRLLE MDGHNQDHLK LFKKEVMNYR QTRHENVVLF MGACMNPPHL AIITSFCKGR TLHSFVRDPK TSLDINKTRQ IAQEIIKGMG YLHAKGIVHK DLKSKNVFYD NGKVVITDFG LFGISGVVRE GRRENQLKLS HDWLCYLAPE IVREMTPGKD EDQLPFSKAA DVYAFGTVWY ELQARDWPLK NQAAEASIWQ IGSGEGMKRV LTSVSLGKEV SEILSACWAF DLQERPSFSL LMDMLEKLPK LNRRLSHPGH FWKSAEINSS KVVPRFERFG LGVLESSNPK M -
Predicted molecular weight
66 kDa including tags -
Amino acids
431 to 921 -
Tags
proprietary tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab190396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 25% Glycerol (glycerin, glycerine), 0.003% EDTA, 0.004% DTT, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- BKSR 1
- BKSR1
- dKsr
see all -
Function
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. -
Cellular localization
Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. - Information by UniProt
Images
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SDS-PAGE - Recombinant human KSR1 protein (ab190396)
SDS-PAGE analysis of ab190396
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Functional Studies - Recombinant human KSR1 protein (ab190396)Kinase assay demonstrating the specific activity of ab190396.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab190396 has not yet been referenced specifically in any publications.
