Recombinant human KSR1 protein (ab190396)

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Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 85% Densitometry
  • Active: Yes
  • Tags: proprietary tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human KSR1 protein
    See all KSR1 proteins and peptides

  • Biological activity

    The specific activity of ab190396 was determined to be 8 nmol/min/mg.

  • Purity

    > 85 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q8IVT5

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      KEHPPAMNHL DSSSNPSSTT SSTPSSPAPF PTSSNPSSAT TPPNPSPGQR DSRFNFPAAY FIHHRQQFIF PVPSAGHCWK CLLIAESLKE NAFNISAFAH AAPLPEAADG TRLDDQPKAD VLEAHEAEAE EPEAGKSEAE DDEDEVDDLP SSRRPWRGPI SRKASQTSVY LQEWDIPFEQ VELGEPIGQG RWGRVHRGRW HGEVAIRLLE MDGHNQDHLK LFKKEVMNYR QTRHENVVLF MGACMNPPHL AIITSFCKGR TLHSFVRDPK TSLDINKTRQ IAQEIIKGMG YLHAKGIVHK DLKSKNVFYD NGKVVITDFG LFGISGVVRE GRRENQLKLS HDWLCYLAPE IVREMTPGKD EDQLPFSKAA DVYAFGTVWY ELQARDWPLK NQAAEASIWQ IGSGEGMKRV LTSVSLGKEV SEILSACWAF DLQERPSFSL LMDMLEKLPK LNRRLSHPGH FWKSAEINSS KVVPRFERFG LGVLESSNPK M

    • Predicted molecular weight

      66 kDa including tags

    • Amino acids

      431 to 921

    • Tags

      proprietary tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab190396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 25% Glycerol (glycerin, glycerine), 0.003% EDTA, 0.004% DTT, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • BKSR 1
    • BKSR1
    • dKsr
    • EK 31
    • EK31
    • hb
    • Kinase suppressor of ras
    • Kinase suppressor of Ras 1
    • KSR
    • KSR 1
    • KSR1
    • KSR1_HUMAN
    • Positive Ras signaling mediator family member
    • Positive Ras signaling mediator family member (86.4 kD)
    • RSU 2
    • RSU2
    • SR 31
    • SR31
    • Suppressor of activated let 60 Ras SUR3
    • Suppressor of Ras1 31
    see all

  • Function

    Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.

  • Cellular localization

    Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human KSR1 protein (ab190396)
    SDS-PAGE - Recombinant human KSR1 protein (ab190396)

    SDS-PAGE analysis of ab190396

  • Functional Studies - Recombinant human KSR1 protein (ab190396)
    Functional Studies - Recombinant human KSR1 protein (ab190396)

    Kinase assay demonstrating the specific activity of ab190396.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab190396? Please let us know so that we can cite the reference in this datasheet.

ab190396 has not yet been referenced specifically in any publications.

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