Description

  • Product name

    Recombinant human KSR2 (mutated R676 S) protein
    See all KSR2 proteins and peptides
  • Biological activity

    Specific activity of ab184730 was determined to be 48 nmol/min/mg as per activity assay protocol.

  • Purity

    > 70 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      RQQKNFN LPASHYYKYK QQFIFPDVVP VPETPTRAPQ VILHPVTSNP ILEGNPLLQI EVEPTSENEE VHDEAEESED DFEEMNLSLL SARSFPRKAS QTSIFLQEWD IPFEQLEIGE LIGKGSFGQV YHGRWHGEVA IRLIDIERDN EDQLKAFKRE VMAYRQTRHE NVVLFMGACM SPPHLAIITS LCKGRTLYSV VRDAKIVLDV NKTRQIAQEI VKGMGYLHAK GILHKDLKSK NVFYDNGKVV ITDFGLFSIS GVLQAGRRED KLRIQNGWLC HLAPEIIRQL SPDTEEDKLP FSKHSDVFAL GTIWYELHAR EWPFKTQPAE AIIWQMGTGM KPNLSQIGMG KEISDILLFC WAFEQEERPT FTKLMDMLEK LPKRNRRLSH PGHFWKSAEL
    • Predicted molecular weight

      72 kDa including tags
    • Amino acids

      554 to 950
    • Modifications

      mutated R676 S
    • Tags

      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab184730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 25% Glycerol, 0.003% EDTA, 0.004% DTT, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FLJ25965
    • hKSR 2
    • hKSR2
    • Kinase suppressor of Ras 2
    • KSR 2
    • Ksr2
    • KSR2_HUMAN
    see all
  • Function

    Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
  • Tissue specificity

    Mainly expressed in brain and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications

    Phosphorylated on Ser-474 by MARK3.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt

Images

  • Kinase assay; The specific activity of ab184730 was determined to be 48 nmol/min/mg as per activity assay protocol.

  • SDS Page analysis of ab184730.

References

ab184730 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184730.
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