Recombinant human KSR2 protein (ab185259)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human KSR2 protein
See all KSR2 proteins and peptides -
Biological activity
The specific activity of ab185259 was determined to be 150 nmol/min/mg.
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Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILE GNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTS IFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQ LKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRD AKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITD FGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSK HSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEI SDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL -
Predicted molecular weight
72 kDa including tags -
Amino acids
554 to 950 -
Tags
proprietary tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab185259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FLJ25965
- hKSR 2
- hKSR2
see all -
Function
Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. -
Tissue specificity
Mainly expressed in brain and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. -
Post-translational
modificationsPhosphorylated on Ser-474 by MARK3. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human KSR2 protein (ab185259)The specific activity of KSR2 (ab185259) was determined to be 135 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human KSR2 protein (ab185259)SDS PAGE analysis of ab185259 -
SDS-PAGE - Recombinant human KSR2 protein (ab185259)SDS PAGE analysis of ab185259 -
SDS-PAGE - Recombinant human KSR2 protein (ab185259)SDS-PAGE analysis of ab185259.
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Functional Studies - Recombinant human KSR2 protein (ab185259)Kinase Assay showing the specific activity of ab185259 as 150 nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab185259 has not yet been referenced specifically in any publications.
