Recombinant human KSR2 protein (ab185259)

Submit a review Submit a question

Description

  • Product name

    Recombinant human KSR2 protein
    See all KSR2 proteins and peptides

  • Biological activity

    The specific activity of ab185259 was determined to be 150 nmol/min/mg.

  • Purity

    > 70 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q6VAB6

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILE GNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTS IFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQ LKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRD AKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITD FGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSK HSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEI SDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

    • Predicted molecular weight

      72 kDa including tags

    • Amino acids

      554 to 950

    • Tags

      proprietary tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab185259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FLJ25965
    • hKSR 2
    • hKSR2
    • Kinase suppressor of Ras 2
    • KSR 2
    • Ksr2
    • KSR2_HUMAN
    see all

  • Function

    Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

  • Tissue specificity

    Mainly expressed in brain and kidney.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.

  • Domain

    The protein kinase domain is predicted to be catalytically inactive.

  • Post-translational
    modifications

    Phosphorylated on Ser-474 by MARK3.

  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human KSR2 protein (ab185259)
    Functional Studies - Recombinant human KSR2 protein (ab185259)
    The specific activity of KSR2 (ab185259) was determined to be 135 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human KSR2 protein (ab185259)
    SDS-PAGE - Recombinant human KSR2 protein (ab185259)
    SDS PAGE analysis of ab185259
  • SDS-PAGE - Recombinant human KSR2 protein (ab185259)
    SDS-PAGE - Recombinant human KSR2 protein (ab185259)
    SDS PAGE analysis of ab185259
  • SDS-PAGE - Recombinant human KSR2 protein (ab185259)
    SDS-PAGE - Recombinant human KSR2 protein (ab185259)

    SDS-PAGE analysis of ab185259.

  • Functional Studies - Recombinant human KSR2 protein (ab185259)
    Functional Studies - Recombinant human KSR2 protein (ab185259)

    Kinase Assay showing the specific activity of ab185259 as 150 nmol/min/mg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab185259? Please let us know so that we can cite the reference in this datasheet.

ab185259 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab185259.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com