Recombinant human KSR2 protein (ab185259)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant human KSR2 protein
See all KSR2 proteins and peptides -
Biological activity
The specific activity of ab185259 was determined to be 150 nmol/min/mg.
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Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILE GNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTS IFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQ LKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRD AKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITD FGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSK HSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEI SDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL -
Predicted molecular weight
72 kDa including tags -
Amino acids
554 to 950 -
Tags
proprietary tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab185259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FLJ25965
- hKSR 2
- hKSR2
see all -
Function
Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. -
Tissue specificity
Mainly expressed in brain and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. -
Post-translational
modificationsPhosphorylated on Ser-474 by MARK3. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human KSR2 protein (ab185259)The specific activity of KSR2 (ab185259) was determined to be 135 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human KSR2 protein (ab185259)SDS PAGE analysis of ab185259 -
SDS-PAGE - Recombinant human KSR2 protein (ab185259)SDS PAGE analysis of ab185259 -
SDS-PAGE - Recombinant human KSR2 protein (ab185259)SDS-PAGE analysis of ab185259.
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Functional Studies - Recombinant human KSR2 protein (ab185259)Kinase Assay showing the specific activity of ab185259 as 150 nmol/min/mg.
Datasheets and documents
References
ab185259 has not yet been referenced specifically in any publications.
