Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human KSR2 protein
See all KSR2 proteins and peptides
The specific activity of ab185259 was determined to be 150 nmol/min/mg.
Purity> 70 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILE GNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTS IFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQ LKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRD AKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITD FGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSK HSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEI SDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL
Predicted molecular weight72 kDa including tags
Amino acids554 to 950
Tagsproprietary tag N-Terminus
Our Abpromise guarantee covers the use of ab185259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- hKSR 2
FunctionLocation-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
Tissue specificityMainly expressed in brain and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
modificationsPhosphorylated on Ser-474 by MARK3.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab185259 has not yet been referenced specifically in any publications.