Description

  • Product name

    Recombinant Human Ku70 + Ku80 protein
  • Purity

    > 90 % SDS-PAGE.
    This protein is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Additional sequence information

      KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab73790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3808% HEPES, 20% Glycerol, 0.928% Sodium chloride

General Info

  • Alternative names

    • CTC75
    • CTC85
    • CTCBF
    • DNA repair protein XRCC5
    • DNA repair protein XRCC6
    • G22P1
    • G22P2
    • KARP1
    • Ku 70
    • Ku 80
    • Ku 86
    • Ku86
    • ML8
    • NFIV
    • TLAA
    • XRCC5
    • XRCC6
    see all
  • Relevance

    The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
  • Cellular localization

    Nuclear

References

ab73790 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry. A suitable detection antibody could be against the His tag. For example HRP conjugated: ab1187 ab1269 ab3553 Detection of Ku70/Ku80 heterodimer by Ku antibodies may bear false-negative results since the main reactive epitope is conformation-dependent. The only WB tested antibody against Ku70/Ku80 heterodimer is ab53126. I hope this information is helpful to you.

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