Description

  • Product name

    Recombinant Human L3MBTL1 protein (His tag)
    See all L3MBTL1 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      EWSSSQPATG EKKECWSWES YLEEQKAITA PVSLFQDSQA VTHNKNGFKL GMKLEGIDPQ HPSMYFILTV AEVCGYRLRL HFDGYSECHD FWVNANSPDI HPAGWFEKTG HKLQPPKGYK EEEFSWSQYL RSTRAQAAPK HLFVSQSHSP PPLGFQVGMK LEAVDRMNPS LVCVASVTDV VDSRFLVHFD NWDDTYDYWC DPSSPYIHPV GWCQKQGKPL TPPQDYPDPD NFCWEKYLEE TGASAVPTWA FKVRPPHSFL VNMKLEAVDR RNPALIRVAS VEDVEDHRIK IHFDGWSHGY DFWIDADHPD IHPAGWCSKT GHPLQPPLGP REPSSASPGG
    • Predicted molecular weight

      40 kDa including tags
    • Amino acids

      191 to 530
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab216180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 20% Glycerol, 0.02% Potassium chloride, 0.64% Sodium chloride, 0.63% Tris HCl

General Info

  • Alternative names

    • dJ138B7.3
    • H-L(3)MBT
    • H-l(3)mbt protein
    • KIAA0681
    • L(3)mbt protein homolog
    • L(3)mbt-like
    • L3MBT
    • L3MBTL
    • L3MBTL1
    • Lethal (3) malignant brain tumor l(3
    • Lethal(3)malignant brain tumor-like protein 1
    • LMBL1_HUMAN
    • RGD1307316
    • ZC2HC3
    see all
  • Function

    Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
  • Tissue specificity

    Widely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60.
  • Sequence similarities

    Contains 1 C2HC-type zinc finger.
    Contains 3 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Developmental stage

    In interphase cells, it is scattered throughout the nucleoplasm. In mitotic cells, it strongly associates with condensed chromosomes from the prophase to telophase.
  • Domain

    The MBT repeat 2 specifically recognizes and binds monomethylated and dimethylated proteins. In contrast, it does not bind trimethylated proteins. The MBT repeat 1 does not bind methylated peptides but inserts a proline ring in a Pro-Ser-Ser/Thr sequence context.
  • Cellular localization

    Nucleus. Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex.
  • Information by UniProt

Images

  • 4-20% SDS-PAGE analysis of 2 μg ab216180

References

ab216180 has not yet been referenced specifically in any publications.

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