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    recombinant-human-lair2-protein-ab182705.pdf

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Immunology Immunoglobulins Receptors
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Bioactive grade

Recombinant human LAIR2 protein (ab182705)

  • Datasheet
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SDS-PAGE - Recombinant human LAIR2 protein (ab182705)
  • Functional Studies - Recombinant human LAIR2 protein (ab182705)
  • SDS-PAGE - Recombinant human LAIR2 protein (ab182705)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human LAIR2 protein
    See all LAIR2 proteins and peptides
  • Biological activity

    Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells.
    When 10000 cells/well are added to ab182705 coated plates (50 μg/ml with 100 μl/well), approximately >40% cells will adhere after 10 minutes at 37°C.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    Q6ISS4
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      QEGALPRPSISAEPGTVISPGSHVTFMCRGPVGVQTFRLEREDRAKYKDS YNVFRLGPSESEARFHIDSVSEGNAGLYRCLYYKPPGWSEHSDFLELLVK ESSGGPDSPDTEPGSSAGTVPGTEASGFDAP
    • Predicted molecular weight

      15 kDa including tags
    • Amino acids

      22 to 152
    • Tags

      His tag C-Terminus
    • Additional sequence information

      AAH69366

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-LAIR2 antibody (ab183145)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab182705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • CD306
    • CD306 antigen
    • LAIR 2
    • LAIR-2
    • LAIR2
    • LAIR2_HUMAN
    • Leukocyte associated Ig like receptor 2
    • Leukocyte associated immunoglobulin like receptor 2
    • Leukocyte-associated immunoglobulin-like receptor 2
    • MGC71634
    • OTTHUMP00000068389
    • OTTHUMP00000068390
    see all
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q6ISS4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human LAIR2 protein (ab182705)
    SDS-PAGE - Recombinant human LAIR2 protein (ab182705)
    Human LAIR-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
  • Functional Studies - Recombinant human LAIR2 protein (ab182705)
    Functional Studies - Recombinant human LAIR2 protein (ab182705)
    Immobilized Human Collagen I protein at 2 µg/mL (100 µL/well) can bind Human LAIR-2, His Tag with a linear range of 5-78 ng/mL.
  • SDS-PAGE - Recombinant human LAIR2 protein (ab182705)
    SDS-PAGE - Recombinant human LAIR2 protein (ab182705)

    DTT-reduced SDS-PAGE analysis of ab182705 stained overnight with Coomassie Blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab182705? Please let us know so that we can cite the reference in this datasheet.

    ab182705 has been referenced in 1 publication.

    • Fu Q  et al. Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte-associated immunoglobulin-like receptor 1. Mol Med Rep 16:2791-2798 (2017). PubMed: 28677766

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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